Biotransformation of ethyl 2-(2′-nitrophenoxy)acetate to benzohydroxamic acid (D-DIBOA) by Escherichia coli

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Biotransformation of ethyl 2-(2′-nitrophenoxy)acetate to benzohydroxamic acid (D-DIBOA) by Escherichia coli

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dc.contributor.author Valle, Antonio
dc.contributor.author Cabrera Revuelta, Gema
dc.contributor.author Molinillo, Jose Maria
dc.contributor.author Gómez Montes de Oca, José Manuel
dc.contributor.author Macías Domínguez, Francisco Antonio
dc.contributor.author Cantero, Domingo
dc.contributor.other Ingeniería Química y Tecnología de Alimentos en_US
dc.date.accessioned 2014-05-23T08:11:16Z
dc.date.available 2014-05-23T08:11:16Z
dc.date.issued 2011-01-01T00:00:00Z
dc.identifier.issn 1359-5113
dc.identifier.uri http://hdl.handle.net/10498/16310
dc.description http://dx.doi.org/10.1016/j.procbio.2010.09.011 en_US
dc.description.abstract Benzohydroxamic acids, such as DIBOA, exhibit interesting biological properties (herbicidal, fungicidal and bactericidal). Recently, the synthesis of DIBOA has been simplified to only two steps. This paper explores the possibility of replacing the second stage in the chemical synthesis of D-DIBOA by a biotransformation using a strain of Escherichia coli and a strain of Serratia marcescens. Biotransformation experiments were carried out for both strains in the presence of different concentrations (0.25, 0.5 and 1 mg/mL) of the precursor (ethyl 2-(2 -nitrophenoxy)acetate) under aerobic and anaerobic conditions. Both strains tolerated the concentrations of precursor investigated here. Higher biotransformation yields were reached for E. coli under aerobic conditions. The UV/vis spectra and 1H/13C spectroscopic data obtained from HPLC-DAD and NMR, respectively, for the compounds obtained in the biotransformation reaction confirmed the presence of D-DIBOA in cultures of E. coli. The maximum yields were obtained in experiments supplemented with 0.5mg/mL of precursor and these were 20.14±1.87% under aerobic conditions and 8.17±0.94% under anaerobic conditions. en_US
dc.format application/pdf
dc.language.iso eng en_US
dc.publisher Elsevier en_US
dc.rights info:eu-repo/semantics/openAccess
dc.source Process Biochemistry - 2011 - Volumen 46, n. 1 pp. 358–364 en_US
dc.subject Biotransformation en_US
dc.subject Escherichia coli en_US
dc.subject Serratia marcescens en_US
dc.subject Benzohydroxamic acid en_US
dc.subject Natural product en_US
dc.title Biotransformation of ethyl 2-(2′-nitrophenoxy)acetate to benzohydroxamic acid (D-DIBOA) by Escherichia coli en_US
dc.type info:eu-repo/semantics/article en_US
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.identifier.doi 10.1016/j.procbio.2010.09.011
dc.identifier.doi 10.1016/j.procbio.2010.09.011

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