The botrydial biosynthetic gene cluster of Botrytis cinerea displays a bipartite genomic structure and is positively regulated by the putative Zn(II)2Cys6 transcription factor BcBot6

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The botrydial biosynthetic gene cluster of Botrytis cinerea displays a bipartite genomic structure and is positively regulated by the putative Zn(II)2Cys6 transcription factor BcBot6

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dc.contributor.author Porquier, Antoine
dc.contributor.author Morgant, Guillaume
dc.contributor.author Moraga Galindo, Javier
dc.contributor.author Dalmais, Bérengère
dc.contributor.author Luyten, Isabelle
dc.contributor.author Simon, Adeline
dc.contributor.author Pradier, Jean-Marc
dc.contributor.author González Collado, Isidro
dc.contributor.author Amselem, Joëlle
dc.contributor.author Viaud, Muriel
dc.contributor.other Química Orgánica en_US
dc.date.accessioned 2018-06-06T07:31:10Z
dc.date.available 2018-06-06T07:31:10Z
dc.date.issued 2016
dc.identifier.uri http://hdl.handle.net/10498/20596
dc.description.abstract Botrydial (BOT) is a non-host specific phytotoxin produced by the polyphagous phytopathogenic fungus Botrytis cinerea. The genomic region of the BOT biosynthetic gene cluster was investigated and revealed two additional genes named Bcbot6 and Bcbot7. Analysis revealed that the G + C/A + T-equilibrated regions that contain the Bcbot genes alternate with A + T-rich regions made of relics of transposable elements that have undergone repeat-induced point mutations (RIP). Furthermore, BcBot6, a Zn(II)2Cys6 putative transcription factor was identified as a nuclear protein and the major positive regulator of BOT biosynthesis. In addition, the phenotype of the DBcbot6 mutant indicated that BcBot6 and therefore BOT are dispensable for the development, pathogenicity and response to abiotic stresses in the B. cinerea strain B05.10. Finally, our data revealed that B. pseudocinerea, that is also polyphagous and lives in sympatry with B. cinerea, lacks the ability to produce BOT. Identification of BcBot6 as the major regulator of BOT synthesis is the first step towards a comprehensive understanding of the complete regulation network of BOT synthesis and of its ecological role in the B. cinerea life cycle. en_US
dc.format application/pdf en_US
dc.language.iso eng en_US
dc.publisher Elsevier en_US
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 Internacional *
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/ *
dc.source Fungal Genetics and Biology 96 (2016) 33–46 en_US
dc.subject Botrytis cinerea en_US
dc.subject Secondary metabolism en_US
dc.subject Toxin en_US
dc.subject Botrydial en_US
dc.subject Transcription factor en_US
dc.subject Zn(II)2Cys6 en_US
dc.title The botrydial biosynthetic gene cluster of Botrytis cinerea displays a bipartite genomic structure and is positively regulated by the putative Zn(II)2Cys6 transcription factor BcBot6 en_US
dc.type info:eu-repo/semantics/article en_US
dc.identifier.doi 10.1016/j.fgb.2016.10.003

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Attribution-NonCommercial-NoDerivatives 4.0 Internacional Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 Internacional