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dc.contributor.authorGarcía, Emilio
dc.contributor.authorCross, Ismael
dc.contributor.authorPortela-Bens, Silvia
dc.contributor.authorRodríguez Jiménez, María Esther
dc.contributor.authorGarcía-Angulo, Aglaya
dc.contributor.authorMolina, Belén
dc.contributor.authorCuadrado, Angeles
dc.contributor.authorLiehr, Thomas
dc.contributor.authorRebordinos, Laureana
dc.contributor.otherBioquímica y Biología Molecular, Microbiología, Medicina Preventiva, Salud Públicaen_US
dc.date.accessioned2019-12-11T11:54:34Z
dc.date.available2019-12-11T11:54:34Z
dc.date.issued2019-11
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/10498/21969
dc.description.abstractRepetitive sequences play an essential role in the structural and functional evolution of the genome, particularly in the sexual chromosomes. The Senegalese sole (Solea senegalensis) is a valuable flatfish in aquaculture albeit few studies have addressed the mapping and characterization of repetitive DNA families. Here we analyzed the Simple Sequence Repeats (SSRs) and Transposable elements (TEs) content from fifty-seven BAC clones (spanning 7.9 Mb) of this species, located in chromosomes by multiple fluorescence in situ hybridization (m-BAC-FISH) technique. The SSR analysis revealed an average density of 675.1 loci per Mb and a high abundance (59.69%) of dinucleotide coverage was observed, being 'AC' the most abundant. An SSR-FISH analysis using eleven probes was also carried out and seven of the 11 probes yielded positive signals. 'AC' probes were present as large clusters in almost all chromosomes, supporting the bioinformatic analysis. Regarding TEs, DNA transposons (Class II) were the most abundant. In Class I, LINE elements were the most abundant and the hAT family was the most represented in Class II. Rex/Babar subfamily, observed in two BAC clones mapping to chromosome pair 1, showed the longest match. This chromosome pair has been recently reported as a putative sexual proto-chromosome in this species, highlighting the possible role of the Rex element in the evolution of this chromosome. In the Rex1 phylogenetic tree, the Senegalese sole Rex1 retrotransposon could be associated with one of the four major ancient lineages in fish genomes, in which it is included O. latipes.en_US
dc.formatapplication/pdfen_US
dc.language.isoengen_US
dc.publisherNATURE PUBLISHING GROUPen_US
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.sourceScientific Reports volume 9, Article number: 17146 (2019)en_US
dc.titleIntegrative genetic map of repetitive DNA in the sole Solea senegalensis genome shows a Rex transposon located in a proto-sex chromosomeen_US
dc.typeinfo:eu-repo/semantics/articleen_US
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen_US
dc.identifier.doi10.1038/s41598-019-53673-6


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Atribución 4.0 Internacional
This work is under a Creative Commons License Atribución 4.0 Internacional