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dc.contributor.authorFernández-Cuenca, Felipe
dc.contributor.authorPérez-Palacios, Patricia
dc.contributor.authorGalán-Sánchez, Fátima
dc.contributor.authorLópez-Cerero, Lorena
dc.contributor.authorLópez-Hernández, Inmaculada
dc.contributor.authorLópez Rojas, Rafael
dc.contributor.authorArca-Suárez, Jorge
dc.contributor.authorDíaz-de Alba, Paula
dc.contributor.authorRodríguez-Iglesias, Manuel A.
dc.contributor.authorPascual, Alvaro
dc.contributor.otherBiomedicina, Biotecnología y Salud Públicaes_ES
dc.date.accessioned2020-05-25T10:54:59Z
dc.date.available2020-05-25T10:54:59Z
dc.date.issued2020-01
dc.identifier.issn0213-005X
dc.identifier.issn1578-1852
dc.identifier.urihttp://hdl.handle.net/10498/23058
dc.description.abstractIntroduction: NDM-1 carbapenemase is spreading rapidly all over the world, but this metallo-beta-lactamase has just been detected for the first time in an Acinetobacter baumannii (Ab) isolate of the ST85 clone in Spain. The aim of this study was to characterize a NDM-1-producing carbapenem-resistant A. baumannii (CR-Ab) isolate submitted to the Andalusian PIRASOA [infection prevention program] referral laboratory. Methods: Carbapenemases were detected by PCR and Sanger DNA sequencing. Whole genome sequencing was performed by NGS (Miseq, Illumina). Resistance genes were identified with RESfinder, while MLSTfinder was used for sequence typing (ST). The genetic location of bla(NDM-1) was determined by nuclease S-1/PFGE/hybridization with specific probe. Results: The isolate was susceptible to amikacin and tigecycline and belonged to the ST85 clone. bla(OXA-94) and bla(NDM-1) were identified by PCR and Sanger DNA sequencing, respectively. The resistance genes aadB, bla(ADC-25), bla(NDM-1), bla(OXA-94), msr(E), mph(E) and floR, sul2 were identified by NGS. The chromosome of the isolate contained a defective Tn125 transposon with bla(NDM-1) flanked by the insertion sequences ISAbA125 and ISAba14. The bla(NDM-1) gene was only detected in the chromosomal DNA. Conclusion: This is the first time that bla(NDM-1) has been detected and characterized in a bla(OXA-94)-producing CR-Ab isolate belonging to the ST85 clone in Spaines_ES
dc.formatapplication/pdfes_ES
dc.language.isoenges_ES
dc.publisherELSEVIERes_ES
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.sourceEnferm Infecc Microbiol Clin. 2020;38(1):11–15es_ES
dc.subjectA. baumanniies_ES
dc.subjectNDM-1es_ES
dc.subjectOXA-94es_ES
dc.subjectST85es_ES
dc.titleFirst identification of bla(NDM-1) carbapenemase in bla(OXA-94)-producing Acinetobacter baumannii ST85 in Spaines_ES
dc.title.alternativeDetección por primera vez en España de la carbapenemasa bla(NDM-1 )en Acinetobacter baumannii ST85 productor de bla (OXA-94)es_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES
dc.identifier.doi10.1016/j.eimc.2019.03.008


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