First approaches to valorizate Fat, Oil and Grease (FOG) as anaerobic co-substrate with slaughterhouse wastewater: biomethane potential, settling capacity and microbial dynamics.

Abstract

Anaerobic digestion (AD) is the biological preferred treatment applied to Slaughterhouse wastewaters (SWW) due to its effectiveness. The aim of the study is to investigate the effect of different percentages of fats, oil and grease (FOG) on biomethane production in anaerobic co-digestion with slaughterhouse wastewater using BMP tests under mesophilic conditions (35 ºC). For this purpose, three percentages of FOG from 1% to 10% were tested. Biodegradability, biomethane production and the microbial population were studied. In addition, settling capacity has been evaluated at different conditions: i) before and after anaerobic co-digestion; ii) at different temperature 25 ºC and 35 ºC. The settling rates as well as the characterization of the digestate were recorded. Experimental results showed that all the co-digestion mixtures (FOG percentages = 1-10%) enhanced biomethane production and biodegradability compared to AD of sole SWW. The best conditions were achieved at 5-10% of FOG, showing biodegradability of 66-70% CODtremoval and specific biomethane productions of 562 and 777 mLCH4·g-1CODsremoved, respectively. Regarding microbial dynamics, Eubacteria was reduced with the increase in %FOG but Acetate utilizing methanogens was increased. Regarding settling capacity, mesophilic temperatures (35 ºC) increased the settling rate of digestate in 1.76 times and reduced the lag-phase to 0.92 min; obtaining a more concentrated sludge and leaving a clarified whose TSS represent only 8% of TS.