RT doctoral thesis
T1 Nuevos métodos para la determinación de isoflavonas en soja y alimentos derivados
T2 New methods for the determination of isoflavones from soy and derived foods
A1 Rostagno, Mauricio Ariel
A2 Química Analítica
K1 chemistry, analytical
K1 agriculture, food science and technology
K1 engineering, chemical
K1 engineering, agricultural
K1 química analítica
K1 agricultura
AB In this Doctoral Thesis several aspects of soy isoflavones determination were assessed for the development of new methods for the extraction, clean-up/concentration and hydrolysis, as well as new analysis methods by high performance chromatography methods. Previously, the stability of soy isoflavones extracts and standard solutions was evaluated. Thereafter, new extraction methods were developed using the latest techniques and materials available. It was evaluated the use of ultrasounds, pressurized liquids, microwaves and solid phase extraction for the extraction of soy isoflavones from several types of samples. The developed methods are fast, reliable and highly reproducible. New materials were also evaluated, like solid phase extraction polymeric cartridges and the monolithic columns for liquid chromatography. These materials were used for the determination of isoflavones from several soy juices samples with excellent results. Globally, the information provided allows increasing the speed, precision, reproducibility, flexibility and decreasing operational costs of the determination of isoflavones not only from soybeans, but also other matrices, since the developed methods can be potentially used for different types of samples.
SN 9788498280395
YR 2005
FD 2005-01-01T00:00:00Z
LK http://hdl.handle.net/10498/14781
UL http://hdl.handle.net/10498/14781
LA spa
DS Repositorio Institucional de la Universidad de Cádiz
RD 08-nov-2026