RT journal article
T1 Biotransformation of ethyl 2-(2′-nitrophenoxy)acetate to benzohydroxamic acid (D-DIBOA) by Escherichia coli
A1 Valle Gallardo, Antonio
A1 Cabrera Revuelta, Gema
A1 González Molinillo, José María
A1 Gómez Montes de Oca, José Manuel
A1 Macías Domínguez, Francisco Antonio
A1 Cantero Moreno, Domingo
A2 Ingeniería Química y Tecnología de Alimentos
K1 Biotransformation
K1 Escherichia coli
K1 Serratia marcescens
K1 Benzohydroxamic acid
K1 Natural product
AB Benzohydroxamic acids, such as DIBOA, exhibit interesting biological properties (herbicidal, fungicidaland bactericidal). Recently, the synthesis of DIBOA has been simplified to only two steps. This paperexplores the possibility of replacing the second stage in the chemical synthesis of D-DIBOA by a biotransformationusing a strain of Escherichia coli and a strain of Serratia marcescens. Biotransformationexperiments were carried out for both strains in the presence of different concentrations (0.25, 0.5 and1 mg/mL) of the precursor (ethyl 2-(2 -nitrophenoxy)acetate) under aerobic and anaerobic conditions. Bothstrains tolerated the concentrations of precursor investigated here. Higher biotransformation yields werereached for E. coli under aerobic conditions. The UV/vis spectra and 1H/13C spectroscopic data obtainedfrom HPLC-DAD and NMR, respectively, for the compounds obtained in the biotransformation reactionconfirmed the presence of D-DIBOA in cultures of E. coli. The maximum yields were obtained in experimentssupplemented with 0.5mg/mL of precursor and these were 20.14±1.87% under aerobic conditionsand 8.17±0.94% under anaerobic conditions.
PB Elsevier
SN 1359-5113
YR 2011
FD 2011-01-01T00:00:00Z
LK http://hdl.handle.net/10498/16310
UL http://hdl.handle.net/10498/16310
LA eng
NO http://dx.doi.org/10.1016/j.procbio.2010.09.011
DS Repositorio Institucional de la Universidad de Cádiz
RD 08-nov-2026