RT journal article
T1 The Sesquiterpene Synthase from the Botrydial Biosynthetic Gene Cluster of the Phytopathogen Botrytis cinerea
A1 Pinedo Rivilla, Cristina
A1 González Collado, Isidro
A1 Wang, Chieh-Mei
A1 Pradier, Jean-Marc
A1 Dalmais, Bérengère
A1 Choquer, Mathias
A1 Le Pêcheur, Pascal
A1 Morgant, Guillaume
A1 Cane, David
A1 Viaud, Muriel
A2 Química Orgánica
K1 botrytis
K1 gen
AB The fungus Botrytis cinerea is the causal agent of the economicallyimportant gray mold disease that affects more than 200 ornamental and agriculturallyimportant plant species. B. cinerea is a necrotrophic plant pathogen that secretesnonspecific phytotoxins, including the sesquiterpene botrydial and thepolyketide botcinic acid. The region surrounding the previously characterizedBcBOT1 gene has now been identified as the botrydial biosynthetic gene cluster.Five genes including BcBOT1 and BcBOT2 were shown by quantitative reversetranscription-PCR to be co-regulated through the calcineurin signaling pathway. Inactivationof the BcBOT2 gene, encoding a putative sesquiterpene cyclase, abolishedbotrydial biosynthesis, which could be restored by in trans complementation.Inactivation of BcBOT2 also resulted in overproduction of botcinic acid thatwas observed to be strain-dependent. Recombinant BcBOT2 protein convertedfarnesyl diphosphate to the parent sesquiterpene of the botrydial biosyntheticpathway, the tricyclic alcohol presilphiperfolan-8 -ol.
PB American Chemical Society
SN 1554-8929
YR 2008
FD 2008-11-26T00:00:00Z
LK http://hdl.handle.net/10498/17355
UL http://hdl.handle.net/10498/17355
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 12-nov-2026