RT journal article
T1 Bio-guided optimization of the ultrasound-assisted extraction of compounds from Annona glabra L. leaves using the etiolated wheat coleoptile bioassay
A1 Matsumoto, Sadao
A1 Valera Montoya, Rosa María
A1 Palma Lovillo, Miguel
A1 González Molinillo, José María
A1 Lima, Inês S.
A1 García Barroso, Carmelo
A1 Macías Domínguez, Francisco Antonio
A2 Química Orgánica
K1 bio-guided extraction
K1 ultrasound-assisted extraction
K1 annona glabra
K1 allelopathic activity
AB A bio-guided optimization of the extraction of bioactive components from Annona glabra leaves has been developed using the etiolated wheat coleoptile bioassay as the control method. The optimization of an ultrasound-assisted extraction of bioactive compounds using allelopathy results as target values has been carried out for the first time. A two-level fractional factorial experimental design was applied to optimize the ultrasound-assisted extraction. The solvent was the extraction variable that had the most marked effect on the resulting bioactivity of the extracts in the etiolated wheat coleoptile bioassay. Extraction time, extraction temperature and the size of the ultrasonic probe also influenced the bioactivity of the extracts. A larger scale extraction was carried out in the next step in the allelopathic study, i.e., the isolation of compounds from the bioactive extract and chemical characterization by spectroscopic techniques, including NMR. Eight compounds were isolated and identified from the active extracts, namelytwo steroids (β-sistosterol and stigmasterol), five diterpenes with the kaurane skeleton (ent-kaur-16-en-19-oic acid, ent-19-methoxy-19-oxokauran-17-oic acid, annoglabasin B, ent-17-hydroxykaur-15-en-19-oic acid and ent-15β,16β-epoxy-17-hydroxy-kauran-19-oic acid) and the acetogenin asimicin. The most active compound was annoglabasin B, which showed inhibition with values of -95% at 10-3 M, -87% at 5 - 10-4 M and greater than -70% at 10-4 M in the etiolated wheat coleoptile bioassay.
PB Ultrasonics Sonochemistry
YR 2014
FD 2014-02
LK http://hdl.handle.net/10498/20302
UL http://hdl.handle.net/10498/20302
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 21-sep-2026