RT journal article
T1 Simplification of the DPPH assay for estimating the antioxidant activity of wine and wine by-products
A1 Carmona Jiménez, Yolanda
A1 García Moreno, María de Valme
A1 Igartuburu Chinchilla, José Manuel
A1 García Barroso, Carmelo
A2 Química Analítica
A2 Química Orgánica
K1 antioxidant activity
K1 DPPH
K1 Wine
K1 EC20
K1 routine analysis
AB The DPPH assay is one of the most commonly employed methods for measuring antioxidant activity. Even though this method is considered very simple and efficient, it does present various limitations which make it complicated to perform. The range of linearity between the DPPH inhibition percentage and sample concentration has been studied with a view to simplifying the method for characterising samples of wine origin. It has been concluded that all the samples are linear in a range of inhibition below 40%, which allows the analysis to be simplified. A new parameter more appropriate for the simplification, the EC20, has been proposed to express the assay results. Additionally, the reaction time was analysed with the object of avoiding the need for kinetic studies in the method. The simplifications considered offer a more functional method, without significant errors, which could be used for routine analysis.
PB Elsevier
YR 2014
FD 2014-05
LK http://hdl.handle.net/10498/20342
UL http://hdl.handle.net/10498/20342
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 06-nov-2026