RT journal article
T1 The Joint Action of Sesquiterpene Lactones from Leaves as an Explanation for the Activity of Cynara cardunculus
A1 F. García, Benito
A1 Rial Cumbrera, Carlos
A1 Varela Montoya, Rosa María
A1 Torres Martínez, Ascensión
A1 González Molinillo, José María
A1 Macías Domínguez, Francisco Antonio
A2 Química Orgánica
K1 sesquiterpene lactones
K1 cytotoxicity bioassays
K1 binary mixtures
K1 isobole
K1 additive
K1 synergism
K1 antagonist
AB The work described herein is a continuation of a previous study centered on the bioprospect of cardoon (Cynara cardunculus) leaf extracts through the isolation of secondary metabolites with phytotoxic activity. Chromatographic fractionations of the ethyl acetate extract and spectroscopic analysis showed that the majority of the components were sesquiterpene lactones. Of these compounds, aguerin B, grosheimin, and cynaropicrin were very active on etiolated wheat coleoptile, standard target species, and weed growth. The joint action of binary mixtures of these three active sesquiterpene lactones and one nonactive compound (11,13-dihydroxy-8-desoxygrosheimin) was studied. The activities of fixed-ratio mixtures were assessed on wheat coleoptile. The results can be interpreted with respect to a reference model by considering dose−response analyses and isobolograms with linear regression analyses. A total of 17 binary mixtures at different levels of inhibition (ED25, ED50, and ED75) were studied, and predominantly they responded additively (25). Deviations from additivity included seven synergistic responses and two antagonistic responses. The joint action of major sesquiterpene lactones isolated from C. cardunculus can explain the activities observed in extracts and fractions. The results reported here reiterate the utility of the wheat coleoptile bioassay as a quick tool to detect potential synergistic effects in binary mixtures.
PB ACS Publications
YR 2016
FD 2016-08
LK http://hdl.handle.net/10498/20351
UL http://hdl.handle.net/10498/20351
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 08-nov-2026