RT journal article
T1 A New Solid Phase Extraction for the Determination of Anthocyanins in Grapes
A1 Ferreiro González, Marta
A1 Ruiz Rodríguez, Ana
A1 F. Barbero, Gerardo
A1 Carrera Fernández, Ceferino Adrián
A1 Ayuso Vilacides, Jesús
A1 Palma Lovillo, Miguel
A1 García Barroso, Carmelo
A2 Química Analítica
A2 Química Física
K1 solid phase extraction
K1 anthocyanins
K1 phenolics
K1 grapes
AB A method for the concentration and cleaning of red grape extracts prior to the determination of anthocyanins by UPLC-DAD has been developed. This method is of special interest in the determination of phenolic maturity as it allows the analysis of the anthocyanins present in grapes. Several different SPE cartridges were assessed, including both C-18- and vinylbenzene-based cartridges. C-18-based cartridges presented a very low retention for the glucosylated anthocyanidins while vinylbenzene-based cartridges showed excellent retention for these compounds. The optimized method involves the initial conditioning of the cartridge using 10 mL of methanol and 10 mL of water, followed by loading of up to 100 mL of red grape extract. Ten mL of water was used in the washing step and anthocyanins were subsequently eluted using 1.5 mL of acidified methanol at pH 2. This method simplifies the determination of individual anthocyanins as, on the one hand, it cleans the sample of interference and, on the other hand, it increases the concentration to up to 25:1.5. The developed method has been validated with a range of different grapes and it has also been tested as a means of determining the different anthocyanins in grapes with different levels of maturity.
PB Molecules
YR 2014
FD 2014-12
LK http://hdl.handle.net/10498/20356
UL http://hdl.handle.net/10498/20356
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 07-nov-2026