RT journal article
T1 Identification of enhanced hydrogen and ethanol Escherichia coli producer strains in a glycerol‑based medium by screening in single‑knock out mutant collections
A1 Valle Gallardo, Antonio
A1 Cabrera Revuelta, Gema
A1 Cantero Moreno, Domingo
A1 Bolívar Pérez, Jorge
A2 Bioquímica y Biología MolecularMicrobiología, Medicina Preventiva, Salud Pública
A2 Ingeniería Química y Tecnología de Alimentos
K1 Escherichia coli
K1 Ethanol
K1 Glycerol
K1 High throughput
K1 Hydrogen
AB Background: Earth’s climate is warming as a result of anthropogenic emissions of greenhouse gases from fossil fuel combustion. Bioenergy, which includes biodiesel, biohydrogen and bioethanol, has emerged as a sustainable alternative fuel source. For this reason, in recent years biodiesel production has become widespread but this industrycurrently generates a huge amount of glycerol as a by-product, which has become an environmental problem in its own right. A feasible possibility to solve this problem is the use of waste glycerol as a carbon source for microbial transformation into biofuels such as hydrogen and ethanol. For instance, Escherichia coli is a microorganism that cansynthesize these compounds under anaerobic conditions.Results: In this work an experimental procedure was established for screening E. coli single mutants to identify strains with enhanced ethanol and/or H2 productions compared to the wild type strain. In an initial screening of 150single mutants, 12 novel strains (gnd, tdcE, rpiA nanE, tdcB, deoB, sucB, cpsG, frmA, glgC, fumA and gadB) were found to provide enhanced yields for at least one of the target products. The mutations, that improve most significantly the parameters evaluated (gnd and tdcE genes), were combined with other mutations in three engineered E. coli mutant strains in order to further redirect carbon flux towards the desired products.Conclusions: This methodology can be a useful tool to disclose the metabolic pathways that are more susceptible tomanipulation in order to obtain higher molar yields of hydrogen and ethanol using glycerol as main carbon source inmultiple E. coli mutants.
PB Bio Med
YR 2015
FD 2015-06
LK http://hdl.handle.net/10498/20378
UL http://hdl.handle.net/10498/20378
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 21-sep-2026