RT journal article
T1 Nitric oxide synthase-dependent immune response against gram negative bacteria in a crustacean, Litopenaeus vannamei
A1 Rodríguez-Ramos, Tania
A1 Carpio, Yamila
A1 Gómez, Leonardo
A1 Estrada, Mario Pablo
A1 Bolívar Pérez, Jorge
A1 Pendón Meléndez, Carlos
A2 Bioquímica y Biología MolecularMicrobiología, Medicina Preventiva, Salud Pública
K1 Aeromonas hydrophila
K1 Invertebrate immunity
K1 Nitric oxide synthase
K1 Litopenaeus vannamei
K1 NOS
AB Nitric oxide (NO) is a short-lived radical generated by nitric oxide synthases (NOS). NO is involved in a variety of functions in invertebrates, including host defense. In previous studies, we isolated and sequenced for the first time the NOS gene from hemocytes of Panulirus argus, demonstrating the inducibility of this enzyme by lipopolysaccharide in vitro e in vivo. Hyperimmune serum was obtained from rabbits immunized with a P. argus eNOS fragment of 31 kDa produced in Escherichia coli, which specifically detected the recombinant polypeptide and the endogenous NOS from lobster hemocytes by western blotting and immunofluorescence. In the present work, we demonstrate that the hyperimmune serum obtained against P. argus NOS also recognizes Litopenaeus vannamei NOS in hemocytes by western blotting and immunofluorescence. Our data also show that while the hemolymph of L. vannamei has astrong antibacterial activity against the Gram negative bacteria Aeromonas hydrophila, the administration of the anti NOS serum reduce the natural bacterial clearance. These results strongly suggest that NOS is required for the shrimp immune defense toward Gram negative bacteria. Therefore, the monitoring of induction of NOS could be an important tool for testing immunity in shrimp farming.
PB Elsevier
YR 2016
FD 2016-01
LK http://hdl.handle.net/10498/20380
UL http://hdl.handle.net/10498/20380
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 07-nov-2026