RT journal article
T1 Cloning and molecular ontogeny of digestive enzymes in fed and food-deprived developing gilthead seabream (Sparus aurata) larvae
A1 Mata Sotres, José Antonio
A1 Martos Sitcha, Juan Antonio
A1 Yúfera, Manuel
A1 Martínez-Rodríguez, Gonzalo
A1 Astola González, Antonio
A2 Bioquímica y Biología MolecularMicrobiología, Medicina Preventiva, Salud Pública
K1 digestive enzymes
K1 fish larvae
K1 gene expression
K1 gilthead seabream
K1 sparus aurata
AB We have determined the expression pattern of key pancreatic enzymes precursors (trypsinogen, try; chymotrypsinogen, ctrb; phospholipase A2, pla2; bile salt-activated lipase, cel; and α-amylase, amy2a) during the larval stage of gilthead seabream (Sparus aurata) up to 60 days after hatching (dph). Previously, complete sequences of try, cel, and amy2awere cloned and phylogenetically analyzed. One new isoformwas found for cel transcript (cel1b). Expression of all enzyme precursors was detected before the mouth opening. Expression of try and ctrb increased during the first days of development and then maintained high values with some fluctuations during thewhole larval stage. The prolipases pla2 and cel1b increased from first-feeding with irregular fluctuation until the end of the experiment. Contrarily, cel1a maintained lowexpression values duringmost of the larval stage increasing at the end of the period. Nevertheless, cel1a expression was negligible as compared with cel1b. The expression of amy2a sharply increased during the first week followed by a gradual decrease. In addition, a fooddeprivation experiment was performed to find the differences in relation to presence/absence of gut content after the opening of the mouth. The food-deprived larvae died at 10 dph. The expression levels of all digestive enzymes increased up to 7 dph, declining sharply afterwards. This expression pattern up to 7 dphwas the same observed in fed larvae, confirming the genetic programming during the early development.Main digestive enzymes in gilthead seabream larvae exhibited the same expression profiles than other marine fish with carnivorous preferences in their juvenile stages.
PB Elsevier
YR 2015
FD 2015-09
LK http://hdl.handle.net/10498/20382
UL http://hdl.handle.net/10498/20382
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 15-nov-2026