RT journal article
T1 Early genotoxic response and accumulation induced by waterborne copper, lead, and arsenic in European seabass, Dicentrarchus labrax
A1 Canalejo, Antonio
A1 Grandado-Castro, M. Dolores
A1 Cordoba, Francisco
A1 Torronteras, Rafael
A1 Díaz de Alba, Margarita Isabel
A1 Espada Bellido, Estrella
A1 Galindo Riaño, María Dolores
A2 Química Analítica
K1 Arsenic
K1 Copper
K1 Fish
K1 Genotoxicity
K1 Micronuclei
K1 Lead
AB Cu, Pb, and As, which are among the most abundant metals in the aquatic environment, are also among the most health-threatened by causing diverse cellular injuries. The aim of this study was to assess and compare the potentialearly induction of genotoxic effects after waterborne Cu, Pb, and As exposure in European seabass, Dicentrarchus labrax, a commercial widely cultured fish, using the micronucleus (MN) assay in peripheral blood erythrocytes. Fish were exposed under laboratory conditions to nominal solutions ranging 0–10 mg/L for 24 and 96 h. Furthermore, actual metal ion concentrations were measured by inductively coupled plasma atomic emission spectroscopy (ICP-AES) or differential pulse anodic stripping voltammetry (DPASV) in water and four fish tissues differentially related to environmental exposition and metal accumulation, i.e. the gills, liver, muscle, and brain. Dose-dependent increases of micronuclei (MNi) frequency were observed after these very short exposures; based on measured metal concentrations in water, the genotoxic effect ordered as Cu>As>Pb. Significant genotoxic effect at 0.009 mg/L Cu, 0.57 mg/L Pb, and 0.01 mg/L As was seen. For Cu and Pb these are only slightly higher, but for As it isnotably lower than the USEPA criteria of maximum concentration to prevent acute toxicity in aquatic organisms.Furthermore, genotoxicity was differentially related to metal accumulation. MNi frequency correlated positively with the content of Pb in all the organs, with the content of As in liver and gills and only with the content of Cu in the brain. In conclusion, our findings raised environmental concerns because these depicted a genotoxic potential of Cu, Pb, and As after a very short exposure to low but environmentally relevant concentrations, too close to regulatory thresholds. In addition, the MN test in D. labrax could be considered an early biomarker of genotoxicity induced by these metals in fish.
PB Springer
YR 2016
FD 2016-02
LK http://hdl.handle.net/10498/20404
UL http://hdl.handle.net/10498/20404
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 12-nov-2026