RT journal article
T1 BcAtf1, a global regulator, controls various differentiation processes and phytotoxin production in Botrytis cinerea
A1 Temme, Nora
A1 Oeser, Birgitt
A1 Massaroli, Michelli
A1 Heller, Jens
A1 Simon, Adeline
A1 González Collado, Isidro
A1 Viaud, Muriel
A1 Tudzynski, Paul
A2 Química Orgánica
AB Atf1-homologous basic region leucine zipper (bZIP) transcription factors are known to act downstream of the stress-activated mitogen-activated protein kinase (SAPK) cascade in mammals, as well as in several fungi; they regulate the transcription of genes involved in the general stress response. Functional analyses of BcAtf1 in Botrytis cinerea show that it is also connected to the SAPK BcSak1, as it shares several stress response target genes. However, Dbcatf1 mutants are not hypersensitive to osmotic or oxidative stress, as are Dbcsak1 mutants. Both BcSak1 and BcAtf1are regulators of differentiation, but their roles in these processes are almost inverse as, in contrast with Dbcsak1, Dbcatf1 mutants are significantly impaired in conidia production and do not differentiate any sclerotia. They show extremely vigorous growth in axenic culture, with a thick layer of aerial hyphae and a marked increase in colonization efficiency on different host plants and tissues. In addition, the sensitivity to cell wall-interfering agents is increased strongly. Microarray analyses demonstrate that the loss of BcAtf1 leads to extensive transcriptional changes: apart from stress response genes, the expression of a broad set of genes, probably involved in primary metabolism, cell wall synthesis and development, is affected by BcAtf1. Unexpectedly, BcAtf1 also controls secondary metabolism: the mutant contains significantly elevated levels of phytotoxins. These data indicate that BcAtf1 controls a diversity of cellular processes and has broad regulatory functions.
PB BSPP and Blackwell Publishing
YR 2012
FD 2012
LK http://hdl.handle.net/10498/20549
UL http://hdl.handle.net/10498/20549
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 06-nov-2026