RT journal article
T1 Relevance of trichothecenes in fungal physiology: Disruption of tri5 in Trichoderma arundinaceum
A1 G. Malmierca, Mónica
A1 Cardoza, Rosa Elena
A1 J. Alexander, Nancy
A1 McCormick, Susan P.
A1 González Collado, Isidro
A1 Hermosa, Rosa
A1 Monte, Enrique
A1 Gutiérrez, Santiago
A2 Química Orgánica
K1 Trichoderma
K1 Trichothecene
K1 Terpene
K1 Ergosterol
K1 Squalene
K1 Biocontrol
AB Trichothecenes are sesquiterpenoid mycotoxins produced mainly by Fusarium species. Harzianum A (HA), a non-phytotoxic trichothecene produced by Trichoderma arundinaceum, has recently been found to have antagonistic activity against fungal plant pathogens and to induce plant genes involved in defense responses. In the present work, we have shown that disruption of the T. arundinaceum tri5 gene, which encodes a terpene synthase, stops the production of HA, alters the expression of other tri genes involved in HA biosynthesis, and alters the expression of hmgR, dpp1, erg9, erg1, and erg7, all genes involved in terpene biosynthetic pathways. An increase in the level of ergosterol biosynthesis was also observed in the tri5 disrupted transformant in comparison with the wild type strain. The loss of HA also resulted in a drastic reduction of the biocontrol activity of the transformants against the phytopathogenic fungi Botrytis cinerea and Rhizoctonia solani. Finally, the effect of tri5 gene disruption on the regulation and balance of intermediates in terpene biosynthetic pathways, as well as the hypothetical physiological role of trichothecenes, both inter- and intracellularly, on regulation and biocontrol, are discussed.
PB Elsevier
YR 2013
FD 2013
LK http://hdl.handle.net/10498/20571
UL http://hdl.handle.net/10498/20571
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 12-nov-2026