RT journal article
T1 HPLC Analysis of Midodrine and Desglymidodrine in Culture Medium: Evaluation of Static and Shaken Conditions on the Biotransformation by Fungi
A1 Barth, Thiago
A1 Aleu Casatejada, Josefina
A1 Tallarico Pupo, Monica
A1 Sueli Bonato, Pierina
A1 González Collado, Isidro
A2 Química Orgánica
AB A high-performance liquid chromatography (HPLC) method is presented for the simultaneous determination of midodrine and desglymidodrine (DMAE) in Czapek-Dox culture medium, to be used in biotransformation studies by fungi. The HPLC analysis was conducted using a Lichrospher 100 RP18 column, acetonitrile– 40 mmol/L formic acid solution (60:40, v/v) as mobile phase, and ultraviolet detection at 290 nm. The sample preparation was conductedby liquid–liquid extraction using ethyl acetate as extractor solvent. The method was linear over the concentration range of 0.4– 40.0 mg/mL for midodrine (r   0.9997) and DMAE (r   0.9998). Within-day and between-day precision and accuracy were evaluated by relative standard deviations (  8.2%) and relative errors (27.3 to 7.4%), respectively. The validated method was used to assess midodrine biotransformation by the fungi Papulaspora immersa HotsonSS13, Botrytis cinerea UCA 992 and Botrytis cinerea 2100 under static and shaken conditions. Under shaken conditions, the biotransformation of midodrine to DMAE was more efficient for all studied fungi, especially for the fungus Botrytis cinerea 2100, which converted 42.2% of midodrine to DMAE.
PB Oxford University Press
YR 2013
FD 2013
LK http://hdl.handle.net/10498/20579
UL http://hdl.handle.net/10498/20579
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 12-nov-2026