RT journal article
T1 Novel aspinolide production by Trichoderma arundinaceum with a potential role in Botrytis cinerea antagonistic activity and plant defence priming
A1 G. Malmierca, Mónica
A1 Barua, Javier
A1 McCormick, Susan P.
A1 Izquierdo-Bueno Reina, Inmaculada Concepción
A1 Cardoza, Rosa Elena
A1 J. Alexander, Nancy
A1 Hermosa, Rosa
A1 González Collado, Isidro
A1 Monte, Enrique
A1 Gutiérrez, Santiago
A2 Química Orgánica
AB Harzianum A (HA), a trichothecene produced by Trichoderma arundinaceum, has recently been described to have antagonistic activity against fungal plant pathogens and to induce plant defence genes. In the present work, we have shown that a tri5 genedisrupted mutant that lacks HA production overproduces two polyketides, aspinolides B and C, which were not detected in the wild-type strain. Furthermore, four new aspinolides (D–G) were characterized.These compounds confirm that a terpene-polyketide cross-pathway exists in T. arundinaceum, and they may be responsible for the antifungal activity and the plant sensitization effect observed with the tri5- disrupted mutant. In addition, the molecular changes involving virulence factors in the phytopathogenic fungus Botrytis cinerea 98 (Bc98) during interaction with T. arundinaceum were investigated. The expression of genes involved in the production of botrydial by Bc98 was relatively repressed by HA, whereas other virulence genes of this pathogen were inducedby the presence of T. arundinaceum, for example atrB and pg1 which encode for an ABC transporter andendopolygalacturonase 1 respectively. In addition, the interaction with Bc98 significantly repressed the production of HA by T. arundinaceum, indicating that a bidirectional transcriptional regulation is established between these two antagonistic fungi.
PB Willey
YR 2015
FD 2015
LK http://hdl.handle.net/10498/20587
UL http://hdl.handle.net/10498/20587
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 06-nov-2026