RT journal article
T1 Chemically Induced Cryptic Sesquiterpenoids and Expression of Sesquiterpene Cyclases in Botrytis cinerea Revealed New Sporogenic (+)-4-Epieremophil-9-en-11-ols
A1 Pinedo Rivilla, Cristina
A1 Moraga Galindo, Javier
A1 Barua, Javier
A1 González Rodríguez, Victoria Eugenia
A1 Aleu Casatejada, Josefina
A1 Durán Patrón, Rosa María
A1 Macías Sánchez, Antonio José
A1 R. Hanson, James
A1 Viaud, Muriel
A1 Hernández Galán, Rosario
A1 Garrido Crespo, Carlos
A1 González Collado, Isidro
A2 Bioquímica y Biología MolecularMicrobiología, Medicina Preventiva, Salud Pública
A2 Química Orgánica
AB The sequencing of the genomes of the B05.10 and T4 strains of the fungus Botrytis cinerea revealed an abundance of novel biosynthetic gene clusters, the majority of which were unexpected on the basis of the previous analyses ofthe fermentation of these and closely related species. By systematic alteration of easy accessible cultivation parameters, using chemical induction with copper sulfate, we have found a cryptic sesquiterpenoid family with new structures related to eremophil-9-ene, which had the basic structure of the sesquiterpene (+)-5-epiaristolochene ((+)-4-epieremophil-9- ene). An expression study of the sesquiterpene cyclase genes present in the Botrytis cinerea genome, under culture conditions, is reported. In general, a 3 day delay and a higher BcSTC genes expression were observed when copper (5 ppm) was fed to the fermentation broth. In addition, to the observed effect on the BcBOT2 (BcSTC1) gene, involved in the biosynthesis of the botrydial toxin, a higher expression level for BcSTC3 and BcSTC4was observed with respect to the control in the strain B05.10. Interestingly, under copper conditions, the BcSTC4 gene was the most expressed gene in the Botrytis cinerea UCA992 strain. In vitro evaluation of the biological role of these metabolites indicates that they contributed to the conidial development in B. cinerea and appear to be involved in self-regulation of the production of asexual spores. Furthermore, they promoted the formation of complex appressoria or infection cushions.
PB ACS Publications
YR 2016
FD 2016
LK http://hdl.handle.net/10498/20592
UL http://hdl.handle.net/10498/20592
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 06-nov-2026