RT journal article
T1 The botrydial biosynthetic gene cluster of Botrytis cinerea displays a bipartite genomic structure and is positively regulated by the putative Zn(II)2Cys6 transcription factor BcBot6
A1 Porquier, Antoine
A1 Morgant, Guillaume
A1 Moraga Galindo, Javier
A1 Dalmais, Bérengère
A1 Luyten, Isabelle
A1 Simon, Adeline
A1 Pradier, Jean-Marc
A1 González Collado, Isidro
A1 Amselem, Joëlle
A1 Viaud, Muriel
A2 Química Orgánica
K1 Botrytis cinerea
K1 Secondary metabolism
K1 Toxin
K1 Botrydial
K1 Transcription factor
K1 Zn(II)2Cys6
AB Botrydial (BOT) is a non-host specific phytotoxin produced by the polyphagous phytopathogenic fungus Botrytis cinerea. The genomic region of the BOT biosynthetic gene cluster was investigated and revealed two additional genes named Bcbot6 and Bcbot7. Analysis revealed that the G + C/A + T-equilibrated regions that contain the Bcbot genes alternate with A + T-rich regions made of relics of transposable elements that have undergone repeat-induced point mutations (RIP). Furthermore, BcBot6, a Zn(II)2Cys6 putative transcription factor was identified as a nuclear protein and the major positive regulator of BOT biosynthesis. In addition, the phenotype of the DBcbot6 mutant indicated that BcBot6 and therefore BOT are dispensable for the development, pathogenicity and response to abiotic stresses in the B. cinerea strain B05.10. Finally, our data revealed that B. pseudocinerea, that is also polyphagous and lives in sympatry with B. cinerea, lacks the ability to produce BOT. Identification of BcBot6 as the major regulator of BOT synthesis is the first step towards a comprehensive understanding of the complete regulation network of BOT synthesis and of its ecological role in the B. cinerea life cycle.
PB Elsevier
YR 2016
FD 2016
LK http://hdl.handle.net/10498/20596
UL http://hdl.handle.net/10498/20596
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 05-nov-2026