RT journal article
T1 Trichothecenes and aspinolides produced by Trichoderma arundinaceum regulate expression of Botrytis cinerea genes involved in virulence and growth
A1 G. Malmierca, Mónica
A1 Izquierdo-Bueno Reina, Inmaculada Concepción
A1 McCormick, Susan P.
A1 Cardoza, Rosa Elena
A1 J. Alexander, Nancy
A1 Barua, Javier
A1 Lindo, Laura
A1 A. Casquero, Pedro
A1 González Collado, Isidro
A1 Monte, Enrique
A1 Gutiérrez, Santiago
A2 Química Orgánica
AB Trichoderma arundinaceum (Ta37) and Botrytis cinerea (B05.10) produce the sesquiterpenoids harzianum A (HA) and botrydial (BOT), respectively. TaDTri5, an HA non-producer mutant, produces high levels of the polyketide compounds aspinolides (Asp) B and C. We analyzed the role of HA and Asp in the B. cinerea-T. arundinaceum interaction, including changes in BOT production as well as transcriptomic changes of BcBOT genes involved in BOT biosynthesis, and also of genes associated with virulence and ergosterol biosynthesis. We found that exogenouslyadded HA up-regulated the expression of the BcBOT and all the virulence genes analyzed when B. cinerea was grown alone. However, a decrease in the amount of BOT and a down-regulation of BcBOT gene expression was observed in the interaction zone of B05.10- Ta37 dual cultures, compared to TaDTri5. Thus, the confrontation with T. arundinaceum results in an upregulation of most of the B. cinerea genes involved in virulence yet the presence of T. arundinaceum secondary metabolites, HA and AspC, act separately and together to down-regulate the B. cinerea genes analyzed. The present work emphasizes the existence of a chemical cross-regulation between B. cinerea andT. arundinaceum and contributes to understanding how a biocontrol fungus and its prey interact with each other.
PB Willey
YR 2016
FD 2016
LK http://hdl.handle.net/10498/20597
UL http://hdl.handle.net/10498/20597
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 08-nov-2026