RT journal article
T1 Alternative Ultrasound-Assisted Method for the Extraction of the Bioactive Compounds Present in Myrtle (Myrtus communis L.)
A1 Velasco González de Peredo, Ana
A1 Vázquez Espinosa, Mercedes
A1 Espada Bellido, Estrella
A1 Ferreiro González, Marta
A1 Amores Arrocha, Antonio
A1 Palma Lovillo, Miguel
A1 Fernández Barbero, Gerardo
A1 Jiménez Cantizano, Ana Concepción
A2 Ingeniería Química y Tecnología de Alimentos
A2 Química Analítica
K1 anthocyanins
K1 bioactive compounds
K1 Box–Behnken design
K1 ultrasound-assisted extraction
K1 myrtle
K1 Myrtus communis L.
K1 phenolic compounds
AB The bioactive compounds in myrtle berries, such as phenolic compounds and anthocyanins,have shown a potentially positive effect on human health. Efficient extraction methods are to be usedto obtain maximum amounts of such beneficial compounds from myrtle. For that reason, this studyevaluates the effectiveness of a rapid ultrasound-assisted method (UAE) to extract anthocyaninsand phenolic compounds from myrtle berries. The influence of solvent composition, as well as pH,temperature, ultrasound amplitude, cycle and solvent-sample ratio on the total phenolic compoundsand anthocyanins content in the extracts obtained were evaluated. The response variables wereoptimized by means of a Box-Behnken design. It was found that the double interaction of themethanol composition and the cycle, the interaction between methanol composition and temperature,and the interaction between the cycle and solvent-sample ratio were the most influential variables onthe extraction of total phenolic compounds (92.8% methanol in water, 0.2 s of cycle, 60 ◦C and 10:0.5mL:g). The methanol composition and the interaction between methanol composition and pH werethe most influential variables on the extraction of anthocyanins (74.1% methanol in water at pH 7).The methods that have been developed presented high repeatability and intermediate precision (RSD< 5%) and the bioactive compounds show a high recovery with short extraction times. Both methodswere used to analyze the composition of the bioactive compounds in myrtle berries collected fromdifferent locations in the province of Cadiz (Spain). The results obtained by UAE were comparedto those achieved in a previous study where microwave-assisted extraction (MAE) methods wereemployed. Similar extraction yields were obtained for phenolic compounds and anthocyanins byMAE and UAE under optimal conditions. However, UAE presents the advantage of using milderconditions for the extraction of anthocyanins from myrtle, which makes of this a more suitablemethod for the extraction of these degradable compounds.
PB MDPI
SN 1420-3049
YR 2019
FD 2019
LK http://hdl.handle.net/10498/21208
UL http://hdl.handle.net/10498/21208
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 04-nov-2026