RT journal article
T1 The Specialized Roles in Carotenogenesis and Apocarotenogenesis of the Phytoene Synthase Gene Family in Saffron
A1 Ahrazem, Oussama
A1 Diretto, Gianfranco
A1 Argandoña Picazo, Javier
A1 Fiore, Alessia
A1 Rubio-Moraga, Ángela
A1 Rial Cumbrera, Carlos
A1 Varela Montoya, Rosa María
A1 Macías Domínguez, Francisco Antonio
A1 Castillo, Raquel
A1 Romano, Elena
A1 Gómez-Gómez, Lourdes
A2 Química Orgánica
K1 apocarotenoids
K1 carotenoids
K1 phytoene synthase
K1 activity
K1 expression
K1 mycorrhiza
K1 root
K1 stigmas
AB Crocus sativus stigmas are the main source of crocins, which are glucosylatedapocarotenoids derived from zeaxanthin cleavage that give saffron its red color.Phytoene synthase (PSY) mediates the first committed step in carotenoid biosynthesisin plants. Four PSY genes encoding functional enzymes were isolated from saffron.All the proteins were localized in plastids, but the expression patterns of each gene,CsPSY1a, CsPSY1b, CsPSY2, and CsPSY3, in different saffron tissues and during thedevelopment of the stigma showed different tissue specialization. The CsPSY2 transcriptwas primarily detected in the stigmas where it activates and stimulates the accumulationof crocins, while its expression was very low in other tissues. In contrast, CsPSY1aand CsPSY1b were mainly expressed in the leaves, but only CsPSY1b showed stresslightregulation. Interestingly, CsPSY1b showed differential expression of two alternativesplice variants, which differ in the intron retention at their 50 UTRs, resulting in a reductionin their expression levels. In addition, the CsPSY1a and CsPSY1b transcripts, togetherwith the CsPSY3 transcript, were induced in roots under different stress conditions.The CsPSY3 expression was high in the root tip, and its expression was associatedwith mycorrhizal colonization and strigolactone production. CsPSY3 formed a separatebranch to the stress-specific Poaceae homologs but was closely related to the dicotPSY3 enzymes.
PB FRONTIERS MEDIA SA
SN 1664-462X
YR 2019
FD 2019-03
LK http://hdl.handle.net/10498/21299
UL http://hdl.handle.net/10498/21299
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 12-nov-2026