RT journal article
T1 Brain and Pituitary Response to Vaccination in Gilthead Seabream (Sparus aurata L.)
A1 Liu, X. H.
A1 Khansari, A. R.
A1 Teles, M.
A1 Martínez-Rodríguez, Gonzalo
A1 Zhang, Y. G.
A1 Mancera Romero, Juan Miguel
A1 Reyes-Lopez, F. E.
A1 Tort, L.
A2 Biología
K1 brain
K1 pituitary
K1 vaccination
K1 immune response
K1 stress response
AB Vaccination is a widely used therapeutical strategy in aquaculture, but whethervaccination elicits stress responses in the central neuroendocrine system and enhancesthe crosstalk between the immune and endocrine systems in the brain or pituitary aftervaccination is unclear. To answer this question two experiments using two differentvaccine exposure routes, i.e., bath or intraperitoneal (i.p.) injection, were carried outon gilthead seabream (Sparus aurata L.). In the first one, the stress responses of fishsubjected to waterborne Vibrio anguillarum bacterin were compared with responsesafter air exposure or their combination. In the second experiment, fish were subjectedto an intraperitoneal injection of Lactococcus garvieae bacterin and we assessed thecentral stress response and also whether or not a significant immune response wasinduced in brain and pituitary. In both experiments, blood, brain and pituitary tissueswere collected at 1, 6, and 24 h post stress for plasma hormone determination andgene expression analysis, respectively. Results indicated that bath vaccination induceda decreased central stress response compared to air exposure which stimulated bothbrain and pituitary stress genes. In the second experiment, injection vaccination keptunchanged plasma stress hormones except cortisol that raised at 6 and 24 h. Inagreement, non-significant or slight changes on the transcription of stress-related geneswere recorded, including the hormone genes of the hypothalamic pituitary interrenal(HPI) axis and other stress markers such as hsp70, hsp90, and mt genes in either brainor pituitary. Significant changes were observed, however, in crhbp and gr. In this secondexperiment the immune genes il1b, cox2, and lys, showed a strong expression in bothbrain and pituitary after vaccination, notably il1b which showed more than 10 fold raise.Overall, vaccination procedures, although showing a cortisol response, did not induceother major stress response in brain or pituitary, regardless the administration route.Other than main changes, the alteration of crhbp and gr suggests that these genescould play a relevant role in the feedback regulation of HPI axis after vaccination. Inaddition, from the results obtained in this work, it is also demonstrated that the immunesystem maintains a high activity in both brain and pituitary after vaccine injection.
PB FRONTIERS MEDIA SA
SN 1664-042X
YR 2019
FD 2019-06
LK http://hdl.handle.net/10498/21565
UL http://hdl.handle.net/10498/21565
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 21-sep-2026