RT journal article
T1 Proteomic study of the membrane components of signalling cascades of Botrytis cinerea controlled by phosphorylation
A1 Escobar Niño, Almudena
A1 Lineiro, Eva
A1 Amil, Francisco
A1 Carrasco, Rafael
A1 Chiva, Cristina
A1 Fuentes, Carlos
A1 Blanco-Ulate, Barbara
A1 Cantoral Fernández, Jesús Manuel
A1 Sabido, Eduard
A1 Fernández Acero, Francisco Javier
A2 Bioquímica y Biología MolecularMicrobiología, Medicina Preventiva, Salud Pública
K1 unfolded protein response
K1 saccharomyces-cerevisiae
K1 transcription factor
K1 virulence
K1 growth
K1 autophagy
K1 genome
K1 gene
K1 pathogenicity
K1 biosynthesis
AB Protein phosphorylation and membrane proteins play an important role in the infection of plants by phytopathogenic fungi, given their involvement in signal transduction cascades. Botrytis cinerea is a well-studied necrotrophic fungus taken as a model organism in fungal plant pathology, given its broad host range and adverse economic impact. To elucidate relevant events during infection, several proteomics analyses have been performed in B. cinerea, but they cover only 10% of the total proteins predicted in the genome database of this fungus. To increase coverage, we analysed by LC-MS/MS the first-reported overlapped proteome in phytopathogenic fungi, the “phosphomembranome” of B. cinerea, combining the two most important signal transduction subproteomes. Of the 1112 membrane-associated phosphoproteins identified, 64 and 243 were classified as exclusively identified or overexpressed under glucose and deproteinized tomato cell wall conditions, respectively. Seven proteins were found under both conditions, but these presented a specific phosphorylation pattern, so they were considered as exclusively identified or overexpressed proteins. From bioinformatics analysis, those differences in the membrane-associated phosphoproteins composition were associated with various processes, including pyruvate metabolism, unfolded protein response, oxidative stress response, autophagy and cell death. Our results suggest these proteins play a significant role in the B. cinerea pathogenic cycle
PB NATURE PUBLISHING GROUP
SN 2045-2322
YR 2019
FD 2019-07
LK http://hdl.handle.net/10498/21652
UL http://hdl.handle.net/10498/21652
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 15-nov-2026