RT journal article T1 To be or not to be? What molecules say about Runcina brenkoae Thompson, 1980 (Gastropoda: Heterobranchia: Runcinida) A1 Araujo Moreira, Ana Karla A1 Pola, Marta A1 Malaquias, Manuel Antonio E. A1 Cervera, Juan Lucas A2 Biología K1 Runcinida K1 DNA barcoding K1 species delimitation K1 integrative taxonomy K1 biodiversity K1 phylogeny AB Runcinids are poorly known minute marine slugs inhabiting intertidal and shallow subtidal rocky shores. Among the European species, Runcina brenkoae, described from the Adriatic Sea in the Mediterranean, has been described to display chromatic variability, placing in question the true identity and geographic distribution of the species. In this paper we investigate the taxonomic status of R. brenkoae based on specimens from the central and western Mediterranean Sea and the southern Iberian coastline of Portugal and Spain, following an integrative approach combining multi-locus molecular phylogenetics based on the mitochondrial markers cytochrome c oxidase subunit I and 16S rRNA and the nuclear gene histone H3, together with the study of morpho-anatomical characters investigated by scanning electron microscopy. To aid in species delimitation, the Automatic Barcode Gap Discovery and Bayesian Poisson tree process methods were employed. Our results indicate the existence of a complex of three species previously identified as R. brenkoae, namely two new species here described (R. marcosi n. sp. and R. lusitanica n. sp.) and R. brenkoae proper. PB CONSEJO SUPERIOR INVESTIGACIONES CIENTIFICAS-CSIC SN 0214-8358 YR 2019 FD 2019-09 LK http://hdl.handle.net/10498/21794 UL http://hdl.handle.net/10498/21794 LA eng DS Repositorio Institucional de la Universidad de Cádiz RD 13-nov-2026