RT journal article
T1 Contribution of Non-canonical Cortisol Actions in the Early Modulation of Glucose Metabolism of Gilthead Sea Bream (Sparus aurata)
A1 Aedo, Jorge E.
A1 Ruiz Jarabo de la Rocha, Ignacio
A1 Martínez-Rodríguez, Gonzalo
A1 Boltaña, Sebastián
A1 Molina, Alfredo
A1 Valdés, Juan A.
A1 Mancera Romero, Juan Miguel
A2 Biología
K1 cortisol
K1 gene expression
K1 glucose metabolism
K1 membrane-initiated cortisol action
K1 Sparus aurata
K1 stress response
AB Teleost fish are exposed to diverse stressors in farming and wildlife conditions duringtheir lifespan. Cortisol is the main glucocorticoid hormone involved in the regulationof their metabolic acclimation under physiological stressful conditions. In this context,increased plasma cortisol is associated with energy substrate mobilization frommetabolic tissues, such as liver and skeletal muscle, to rapidly obtain energy andcope with stress. The metabolic actions of cortisol have primarily been attributed to itsgenomic/classic action mechanism involving the interaction with intracellular receptors,and regulation of stress-responsive genes. However, cortisol can also interact withmembrane components to activate rapid signaling pathways. In this work, using theteleost fish gilthead sea bream (Sparus aurata) as a model, we evaluated the effects ofmembrane-initiated cortisol actions on the early modulation of glucose metabolism. Forthis purpose, S. aurata juveniles were intraperitoneally administrated with cortisol andwith its membrane impermeable analog, cortisol-BSA. After 1 and 6 h of each treatment,plasma cortisol levels were measured, together with glucose, glycogen and lactate inplasma, liver and skeletal muscle. Transcript levels of corticosteroids receptors (gr1, gr2,and mr) and key gluconeogenesis (g6pc and pepck)- and glycolysis (pgam1 and aldo)related genes in the liver were also measured. Cortisol and cortisol-BSA administrationincreased plasma cortisol levels in S. aurata 1 h after administration. Plasma glucoselevels enhanced 6 h after each treatment. Hepatic glycogen content decreased in theliver at 1 h of both cortisol and cortisol-BSA administration, while increased at 6 h dueto cortisol but not in response to cortisol-BSA. Expression of gr1, g6pc, pgam1, andaldo were preferentially increased by cortisol-BSA in the liver. Taking all these resultsin consideration, we suggest that non-canonical cortisol mechanisms contribute to theregulation of the early glucose metabolism responses to stress in S. aurata.
PB FRONTIERS MEDIA SA
SN 1664-2392
YR 2019
FD 2019-11
LK http://hdl.handle.net/10498/22166
UL http://hdl.handle.net/10498/22166
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 21-sep-2026