RT journal article T1 Biomethane production improvement by enzymatic pretreatments and enhancers of sewage sludge anaerobic digestion A1 Agabo García, Cristina A1 Pérez García, Montserrat A1 Rodríguez-Morgado, Bruno A1 Parrado, Juan A1 Solera del Río, María del Rosario A2 Tecnologías del Medio Ambiente K1 Sewage sludge K1 BMP K1 Enzymatic pre-treatment K1 Anaerobic digestion enhancer K1 Sewage sludge fermentation AB Enzymatic hydrolysis is recognised as an effective pre-treatment for increasing biodegradability of sludge. In this work, isolated commercial enzymes as well as in-situ enzymes producer bacteria were used respectively as enhancers and pre-treatments of sewage sludge. Biodegradability of sample as well as biomethane potential production were studied. Results showed that depuration efficiencies in terms of CODs (73.5-85.5 %) and TVS (28.5-42.7 %) were more than twice the control value. In addition, pre-treated samples as well as enhanced samples with enzymes generated more biomethane than control. The optimal ones, were those with the isolated proteases (P) and with bacteria (Bacillus licheniformis) treatment in-situ (F), producing a total volume of 72.4 ± 2.62 ml CH4 and 114 ml ± 0.46 CH4, respectively, increasing the biogas volume in 3.65 and 5.77 times respectively compared with control. PB ELSEVIER SN 0016-2361 YR 2019 FD 2019-11 LK http://hdl.handle.net/10498/22439 UL http://hdl.handle.net/10498/22439 LA eng DS Repositorio Institucional de la Universidad de Cádiz RD 22-sep-2026