RT journal article
T1 Extraction of anthocyanins and total phenolic compounds from açai (euterpe oleracea mart.) using an experimental design methodology. part 1: Pressurized liquid extraction
A1 Aliaño González, María José
A1 Ferreiro González, Marta
A1 Espada Bellido, Estrella
A1 Carrera Fernández, Ceferino Adrián
A1 Palma Lovillo, Miguel
A1 Álvarez Saura, José Ángel
A1 Ayuso Vilacides, Jesús
A1 Fernández Barbero, Gerardo
A2 Química Analítica
A2 Química Física
K1 açai
K1 anthocyanins
K1 Box–Behnken design
K1 phenolic compounds
K1 pressurized liquid extraction
K1 UHPLC
AB Currently, açai is one of the most important fruits present in the world. Several studies have demonstrated its high content in phenolic compounds and anthocyanins. Both of them are responsible of interesting properties of the fruit such as anti-inflammatory, antioxidant or anticancer. In the present study, two optimized pressurized liquid extraction (PLE) methods have been developed for the extraction of anthocyanins and total phenolic compounds from açai. A full factorial design (Box-Behnken design) with six variables (solvent composition (25%-75% methanol-in-water), temperature (50-100°C), pressure (100-200 atm), purge time (30-90 s), pH (2-7) and flushing (50%-150%)) were employed. The percentage of methanol in the extraction solvent was proven to be the most significant variable for the extraction of anthocyanins. In the case of total phenolic compounds, the extraction temperature was the most influential variable. The developed methods showed high precision, with relative standard deviations (RSD) of less than 5%. The applicability of the methods was successfully evaluated in real samples. In conclusion, two rapid and reliable PLE extraction methods to be used for laboratories and industries to determine anthocyanins and total phenolic compounds in açai and its derived products were developed in this work.
PB MDPI
SN 2073-4395
YR 2020
FD 2020-01
LK http://hdl.handle.net/10498/22732
UL http://hdl.handle.net/10498/22732
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 07-nov-2026