RT journal article
T1 Extraction of Anthocyanins and Total Phenolic Compounds from Açai (Euterpe oleracea Mart.) Using an Experimental Design Methodology. Part 3: Microwave-Assisted Extraction
A1 Aliaño González, María José
A1 Ferreiro González, Marta
A1 Espada Bellido, Estrella
A1 Carrera Fernández, Ceferino Adrián
A1 Palma Lovillo, Miguel
A1 Ayuso Vilacides, Jesús
A1 Fernández Barbero, Gerardo
A1 Álvarez Saura, José Ángel
A2 Química Analítica
A2 Química Física
K1 acai
K1 anthocyanins
K1 Box-Behnken design
K1 microwave-assisted extraction
K1 phenolic compounds
K1 UHPLC
AB In this work, two methods based on microwave-assisted extraction techniques for the extraction of both anthocyanins and total phenolic compounds from acai have been developed. For that, a full factorial design (Box-Behnken design) has been used to optimize the following four variables: solvent composition (25-75% methanol in water), temperature (50-100 degrees C), pH (2-7), and sample/solvent ratio (0.5 g: 10 mL-0.5 g: 20 mL). The anthocyanins and total phenolic compounds content have been determined by ultra high-pressure liquid chromatography and Folin-Ciocalteu method, respectively. The optimum conditions for the extraction of anthocyanins were 38% MeOH in water, 99.63 degrees C, pH 3.00, at 0.5 g: 10 mL of ratio, while for the extraction of total phenolic compounds they were 74.16% MeOH in water, 99.14 degrees C, pH 5.46, at 0.5 g: 20 mL of ratio. Both methods have shown a high repeatability and intermediate precision with a relative standard deviation lower than 5%. Furthermore, an extraction kinetics study was carried out using extraction periods ranging from 2 min until 25 min. The optimized methods have been applied to acai-containing real samples. The results with such real samples have confirmed that both methods are suitable for a rapid and reliable extraction of anthocyanins and total phenolic compounds.
PB MDPI
SN 2073-4395
YR 2020
FD 2020-02
LK http://hdl.handle.net/10498/22766
UL http://hdl.handle.net/10498/22766
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 08-nov-2026