RT journal article
T1 Optimization of Analytical Ultrasound-Assisted Methods for the Extraction of Total Phenolic Compounds and Anthocyanins from Sloes (Prunus spinosa L.)
A1 Velasco González de Peredo, Ana
A1 Vázquez Espinosa, Mercedes
A1 Espada Bellido, Estrella
A1 Ferreiro González, Marta
A1 Carrera Fernández, Ceferino Adrián
A1 Palma Lovillo, Miguel
A1 Álvarez Saura, José Ángel
A1 Fernández Barbero, Gerardo
A1 Ayuso Vilacides, Jesús
A2 Química Analítica
A2 Química Física
K1 anthocyanins
K1 bioactive compounds
K1 Box-Behnken design
K1 phenolic compounds
K1 Prunus spinosaL
K1 sloe
K1 ultra-high-performance liquid chromatography (UHPLC)
K1 ultrasound-assisted extraction
AB Prunus spinosaL. is a shrub that produces berries-sloes-with a high content of phenolic compounds and anthocyanins. Numerous consumer products are made from sloes, such as "Pacharan" liquors or jams. For these reasons, it is crucial to design accurate analytical methods for the extraction of these bioactive compounds in order to promote their consumption and to improve our health. In this study, an ultrasound-assisted extraction method was developed to extract phenolic compounds and anthocyanins from sloes. Several process variables (methanol composition, pH, temperature, cycle, amplitude, and sample-solvent ratio) were optimized based on a Box-Behnken design. The most influential parameters for the extraction of total phenolic compounds were the cycle, the percentage of methanol, and pH of the extraction solvent, with 0.2 s, 67%, and pH 7 as their optimal values, respectively. For total anthocyanins, all parameters were influential, with 44% methanol at pH 2, 48 degrees C, 0.2 s cycles, 30% amplitude, and 1.5 g/20 mL ratio as the optimal values for their corresponding variables. Both methods showed high recoveries, short time use, and high precision (RSD < 5%). In addition, the sloe liqueur "Pacharan" and sloe jams were analyzed to demonstrate the applicability of these methods for the extraction of the real matrix.
PB MDPI
SN 2073-4395
YR 2020
FD 2020-07
LK http://hdl.handle.net/10498/23593
UL http://hdl.handle.net/10498/23593
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 11-nov-2026