RT journal article T1 Physiological and Performance Impacts After Field Supramaximal High-Intensity Interval Training With Different Work-Recovery Duration A1 Albuquerque Brandão, Leandro Henrique A1 Nery Chagas, Thaysa Passos A1 Silva Vasconcelos, Alan Bruno A1 Conceição de Oliveira, Vivian A1 de Sousa Fortes, Leonardo A1 Bezerra de Almeida, Marcos A1 Mendes Netto, Raquel Simões A1 Boscolo Del-Vecchio, Fabrício A1 Pereira Neto, Ezequias A1 Silva Chave, Leury Max A1 Jiménez Pavón, David A1 Da Silva-Grigoletto, Marzo Edir A2 Didáctica de la Educación FísicaPlástica y Musical K1 metabolism K1 biochemistry K1 interval training K1 blood lactate K1 creatine kinase AB High-intensity interval training (HIIT) has numerous external load control variables. The management of these variables makes the physiological responses and performance presented by athletes also modify. The present study aimed to assess the activity of CK and LDH enzymes, performance and metabolic responses caused by two HIIT protocols above the maximum in male recreational runners. Fifteen recreational male runners performed two HIIT protocols in randomized order with multiple conditions: 1) H15 (n= 15), with a HIIT protocol of 15:15 work-recovery duration, and 2) H30 (n= 15) with a HIIT protocol of 30:30 work-recovery duration. Both protocols were performed at similar intensity (130% vV?O-2max), one set until voluntary exhaustion. Blood samples were collected and used to capture the levels and activities of blood lactate (BLac: mmol.L-1), glucose (GLU: mg.dL(-1)), creatine kinase (CK: U.L-1), and lactate dehydrogenase (LDH: U.L-1). BLac and GLU were collected at pre, five, and ten minutes after the H15 and H30 protocols were performed. Blood samples were used to measure the activities of CK and LDH enzymes, which were verified 24 h before and 48 h after the protocols. The distance traveled (m), total time (s), and bouts performed (rep) were also registered. Significant differences between conditions H15 and H30 were observed in the bouts performed (p= 0.001; ES = 1.19). Several statistical differences were found over time for BLac [pre vs. post 5 (both conditions:p= 0.001), pre vs. post 10 (both conditions:p= 0.001), and post 5 vs. post 10 (H30:p= 0.004)], CK [pre vs. post 24 (H15:p< 0.001; ES = 0.97 and H30:p= 0.001; ES = 0.74) post 24 vs. post 48 (H30:p= 0.03; ES = 0.56)], and LDH [pre vs. post24 (H15:p= 0.008; ES = 1.07 and H30:p= 0.022; ES = 0.85). No statistical differences between conditions were observed for any blood parameter. Thus, the volunteers exhibited equal performance in both protocols, which resulted in a similar physiological response. Despite this similarity, in comparison to H15, the H30 protocol presented lower CK activity post 48 and lactate levels after 10 min post protocol. PB FRONTIERS MEDIA SA SN 1664-042X YR 2020 FD 2020-10 LK http://hdl.handle.net/10498/23981 UL http://hdl.handle.net/10498/23981 LA eng DS Repositorio Institucional de la Universidad de Cádiz RD 13-nov-2026