RT journal article
T1 Conversion of Exhausted Sugar Beet Pulp into Fermentable Sugars from a Biorefinery Approach
A1 Marzo Gago, Cristina
A1 Díaz Sánchez, Ana Belén
A1 Caro Pina, Ildefonso
A1 Blandino Garrido, Ana María
A2 Ingeniería Química y Tecnología de Alimentos
K1 enzymatic hydrolysis
K1 solid-state fermentation
K1 sugar beet
K1 sugars hydrolysate
K1 generic microbial feedstock
AB In this study, the production of a hydrolysate rich in fermentable sugars, which could be used as a generic microbial culture medium, was carried out by using exhausted sugar beet pulp pellets (ESBPPs) as raw material. For this purpose, the hydrolysis was performed through the direct addition of the fermented ESBPPs obtained by fungal solid-state fermentation (SSF) as an enzyme source. By directly using this fermented solid, the stages for enzyme extraction and purification were avoided. The effects of temperature, fermented to fresh solid ratio, supplementation of fermented ESBPP with commercial cellulase, and the use of high-solid fed-batch enzymatic hydrolysis were studied to obtain the maximum reducing sugar (RS) concentration and productivity. The highest RS concentration and productivity, 127.3 g.L-1 and 24.3 g.L-1.h(-1) respectively, were obtained at 50 degrees C and with an initial supplementation of 2.17 U of Celluclast (R) per gram of dried solid in fed-batch mode. This process was carried out with a liquid to solid ratio of 4.3 mL.g(-1) solid, by adding 15 g of fermented solid and 13.75 g of fresh solid at the beginning of the hydrolysis, and then the same amount of fresh solid 3 times every 2.5 h. By this procedure, ESBPP can be used to produce a generic microbial feedstock, which contains a high concentration of monosaccharides.
PB MDPI
SN 2304-8158
YR 2020
FD 2020-10
LK http://hdl.handle.net/10498/24062
UL http://hdl.handle.net/10498/24062
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 07-nov-2026