RT journal article
T1 Chitosan-GPTMS-Silica Hybrid Mesoporous Aerogels for Bone Tissue Engineering
A1 Reyes Peces, María Virtudes
A1 Pérez Moreno, Antonio
A1 Santos Martínez, Deseada María de los
A1 Mesa Díaz, María del Mar
A1 Pinaglia Tobaruela, Gonzalo
A1 Vilches Pérez, José Ignacio
A1 Fernández Montesinos, Rafael
A1 Salido Peracaula, Mercedes
A1 Rosa Fox, Nicolás Daniel de la
A1 Piñero de los Ríos, Manuel
A2 Anatomía PatológicaBiología Celular, Histología, Historia de la Ciencia, Medicina Legal y Forense y Toxicología
A2 Física de la Materia Condensada
A2 Ingeniería Química y Tecnología de Alimentos
A2 Materno-Infantil y Radiología
A2 Química Física
K1 hybrid silica aerogels
K1 chitosan
K1 GPTMS
K1 textural properties
K1 mechanical properties
K1 swelling properties
K1 bioactivity
K1 bone tissue engineering
K1 osteoblasts
K1 focal adhesions
AB This study introduces a new synthesis route for obtaining homogeneous chitosan (CS)-silica hybrid aerogels with CS contents up to 10 wt%, using 3-glycidoxypropyl trimethoxysilane (GPTMS) as coupling agent, for tissue engineering applications. Aerogels were obtained using the sol-gel process followed by CO2 supercritical drying, resulting in samples with bulk densities ranging from 0.17 g/cm(3) to 0.38 g/cm(3). The textural analysis by N-2-physisorption revealed an interconnected mesopore network with decreasing specific surface areas (1230-700 m(2)/g) and pore sizes (11.1-8.7 nm) by increasing GPTMS content (2-4 molar ratio GPTMS:CS monomer). In addition, samples exhibited extremely fast swelling by spontaneous capillary imbibition in PBS solution, presenting swelling capacities from 1.75 to 3.75. The formation of a covalent crosslinked hybrid structure was suggested by FTIR and confirmed by an increase of four hundred fold or more in the compressive strength up to 96 MPa. Instead, samples synthesized without GPTMS fractured at only 0.10-0.26 MPa, revealing a week structure consisted in interpenetrated polymer networks. The aerogels presented bioactivity in simulated body fluid (SBF), as confirmed by the in vitro formation of hydroxyapatite (HAp) layer with crystal size of approximately 2 mu m size in diameter. In vitro studies revealed also non cytotoxic effect on HOB(R) osteoblasts and also a mechanosensitive response. Additionally, control cells grown on glass developed scarce or no stress fibers, while cells grown on hybrid samples showed a significant (p < 0.05) increase in well-developed stress fibers and mature focal adhesion complexes.
PB MDPI
SN 2073-4360
YR 2020
FD 2020-11
LK http://hdl.handle.net/10498/24165
UL http://hdl.handle.net/10498/24165
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 05-nov-2026