RT journal article T1 Optimization of a Novel Method Based on Ultrasound-Assisted Extraction for the Quantification of Anthocyanins and Total Phenolic Compounds in Blueberry Samples (Vaccinium corymbosum L.) A1 Aliaño González, María José A1 Jarillo, José Antonio A1 Carrera Fernández, Ceferino Adrián A1 Ferreiro González, Marta A1 Álvarez Saura, José Ángel A1 Palma Lovillo, Miguel A1 Ayuso Vilacides, Jesús A1 Fernández Barbero, Gerardo A1 Espada Bellido, Estrella A2 Química Analítica A2 Química Física K1 anthocyanins K1 antioxidants K1 blueberry K1 Box-Behnken design K1 phenolic compounds K1 response surface methodology K1 ultra-high-performance liquid chromatography K1 UHPLC K1 UV-Vis K1 Vaccinium corymbosum L. AB In recent years, consumers' preference for fruits such as blueberry has increased noticeably. This fact is probably related to their bioactive components such as anthocyanins, phenolic compounds, vitamins, minerals, and tannins that have been found in blueberries by the latest research studies. Both total anthocyanins (TA) and total phenolic compounds (TPC) are known for their multiple beneficial effects on our health, due to their anti-inflammatory, anti-oxidant, and anti-cancer properties. This is the reason why the development of new methodologies for the quality control analysis of raw materials or derived products from blueberry has a great relevance. Two ultrasound-assisted extraction methods (UAE) have been optimized for the quantification of TA and TPC in blueberry samples. The six variables to be optimized were: solvent composition, temperature, amplitude, cycle, extraction solvent pH, and sample/solvent ratio using response surface methodology. The optimized methods have proven to be suitable for the extraction of the TPC and TA with good precision (repeatability and intermediate precision) (coefficient of variation (CV) < 5%) and potentially for application in commercial samples. This fact, together with the multiple advantages of UAE, makes these methods a good alternative to be used in quality control analysis by both industries and laboratories. PB MDPI SN 2304-8158 YR 2020 FD 2020-12 LK http://hdl.handle.net/10498/24351 UL http://hdl.handle.net/10498/24351 LA eng DS Repositorio Institucional de la Universidad de Cádiz RD 11-nov-2026