RT journal article
T1 Hydroxyl Groups Induce Bioactivity in Silica/Chitosan Aerogels Designed for Bone Tissue Engineering. In Vitro Model for the Assessment of Osteoblasts Behavior
A1 Pérez Moreno, Antonio
A1 Reyes Peces, María Virtudes
A1 Santos Martínez, Deseada María de los
A1 Pinaglia Tobaruela, Gonzalo
A1 de la Orden, Emilio
A1 Vilches Pérez, José Ignacio
A1 Salido Peracaula, Mercedes
A1 Piñero de los Ríos, Manuel
A1 Rosa Fox, Nicolás Daniel de la
A2 Anatomía PatológicaBiología Celular, Histología, Historia de la Ciencia, Medicina Legal y Forense y Toxicología
A2 Física de la Materia Condensada
A2 Química Física
K1 aerogels
K1 biomaterials
K1 fracture toughness
K1 hydroxyapatite (HAp)
K1 bone tissue engineering
K1 osteoinduction
AB Silica (SiO2)/chitosan (CS) composite aerogels are bioactive when they are submerged in simulated body fluid (SBF), causing the formation of bone-like hydroxyapatite (HAp) layer. Silica-based hybrid aerogels improve the elastic behavior, and the combined CS modifies the network entanglement as a crosslinking biopolymer. Tetraethoxysilane (TEOS)/CS is used as network precursors by employing a sol-gel method assisted with high power ultrasound (600 W). Upon gelation and aging, gels are dried in supercritical CO2 to obtain monoliths. Thermograms provide information about the condensation of the remaining hydroxyl groups (400-700 degrees C). This step permits the evaluation of the hydroxyl group's content of 2 to 5 OH nm(-2). The formed Si-OH groups act as the inductor of apatite crystal nucleation in SBF. The N-2 physisorption isotherms show a hysteresis loop of type H3, characteristic to good interconnected porosity, which facilitates both the bioactivity and the adhesion of osteoblasts cells. After two weeks of immersion in SBF, a layer of HAp microcrystals develops on the surface with a stoichiometric Ca/P molar ratio of 1.67 with spherulite morphology and uniform sizes of 6 mu m. This fact asserts the bioactive behavior of these hybrid aerogels. Osteoblasts are cultured on the selected samples and immunolabeled for cytoskeletal and focal adhesion expression related to scaffold nanostructure and composition. The initial osteoconductive response observes points to a great potential of tissue engineering for the designed composite aerogels.
PB MDPI
SN 2073-4360
YR 2020
FD 2020-12
LK http://hdl.handle.net/10498/24366
UL http://hdl.handle.net/10498/24366
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 10-nov-2026