RT journal article
T1 Development of New Antiproliferative Compound against Human Tumor Cells from the Marine Microalgae Nannochloropsis gaditana by Applied Proteomics
A1 Carrasco Reinado, Rafael
A1 Escobar Niño, Almudena
A1 Morano, Ines M.
A1 Amil Ruiz, Francisco
A1 Martínez-Rodríguez, Gonzalo
A1 Fuentes Almagro, Carlos
A1 Capilla, Victoria
A1 Tomás Cobos, Lidia
A1 Soriano Romaní, Laura
A1 Guarnizo, Palmira
A1 Vallejo, Roberto A.
A1 Fernández Acero, Francisco Javier
A1 Fajardo Quiñones, Carlos
A2 BiomedicinaBiotecnología y Salud Pública
K1 microalgae; tumor-antiproliferative; protein; biomedicine; applied proteomics; industrial application
K1 microalgae
K1 tumor-antiproliferative
K1 protein
K1 biomedicine
K1 applied proteomics
K1 industrial application
AB Proteomics is a crucial tool for unravelling the molecular dynamics of essential biological processes, becoming a pivotal technique for basic and applied research. Diverse bioinformatic tools are required to manage and explore the huge amount of information obtained from a single proteomics experiment. Thus, functional annotation and protein-protein interactions are evaluated in depth leading to the biological conclusions that best fit the proteomic response in the system under study. To gain insight into potential applications of the identified proteins, a novel approach named "Applied Proteomics" has been developed by comparing the obtained protein information with the existing patents database. The development of massive sequencing technology and mass spectrometry (MS/MS) improvements has allowed the application of proteomics nonmodel microorganisms, which have been deeply described as a novel source of metabolites. Between them, Nannochloropsis gaditana has been pointed out as an alternative source of biomolecules. Recently, our research group has reported the first complete proteome analysis of this microalga, which was analysed using the applied proteomics concept with the identification of 488 proteins with potential industrial applications. To validate our approach, we selected the UCA01 protein from the prohibitin family. The recombinant version of this protein showed antiproliferative activity against two tumor cell lines, Caco2 (colon adenocarcinoma) and HepG-2 (hepatocellular carcinoma), proving that proteome data have been transformed into relevant biotechnological information. From Nannochloropsis gaditana has been developed a new tool against cancer-the protein named UCA01. This protein has selective effects inhibiting the growth of tumor cells, but does not show any effect on control cells. This approach describes the first practical approach to transform proteome information in a potential industrial application, named "applied proteomics". It is based on a novel bioalgorithm, which is able to identify proteins with potential industrial applications. From hundreds of proteins described in the proteome of N. gaditana, the bioalgorithm identified over 400 proteins with potential uses; one of them was selected as UCA01, "in vitro" and its potential was demonstrated against cancer. This approach has great potential, but the applications are potentially numerous and undefined.
PB MDPI
SN 1422-0067
YR 2021
FD 2021-01
LK http://hdl.handle.net/10498/24414
UL http://hdl.handle.net/10498/24414
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 08-nov-2026