RT journal article
T1 HPLC-DAD-MS and Antioxidant Profile of Fractions from Amontillado Sherry Wine Obtained Using High-Speed Counter-Current Chromatograph
A1 Schwarz Rodriguez, Mónica
A1 Weber, Fabian
A1 Durán Guerrero, Enrique
A1 Castro Mejías, Remedios
A1 Rodríguez Dodero, María del Carmen
A1 García Moreno, María de Valme
A1 Winterhalter, Peter
A1 Guillén Sánchez, Dominico Antonio
A2 BiomedicinaBiotecnología y Salud Pública
A2 Química Analítica
K1 HSCCC
K1 sherry wine
K1 Amontillado
K1 phenolic compounds
K1 antioxidant activity
AB n the present work, the polyphenolic profile of a complex matrix such as Amontillado sherry has been processed by means of high-speed counter-current chromatography (HSCCC) and characterized by HPLC-DAD-MS. An Amberlite XAD-7 column was used to obtain the wine extract, and three different biphasic solvent systems were applied for HSCCC separation: MTBE (methyl tert-butyl ether)/n-butanol/acetonitrile/water (1.1/3/1.1/5+0.1% trifluoroacetic acid), MTBE/n-butanol/acetonitrile/water (2/2/1/5), and hexane/ethyl acetate/ethanol/water (1/5/1/5). As a result, 42 phenolic compounds and furanic derivatives have been identified by means of HPLC-DAD-MS, with 11 of them being identified for the first time in Sherry wines: 3-feruloylquinic acid, isovanillin, ethyl vanillate, furoic acid, dihydro-p-coumaric acid, 6-O-feruloylglucose, ethyl gallate, hydroxytyrosol, methyl protocatechuate, homoveratric acid and veratraldehyde. In addition, the antioxidant capacity (ABTS) of the obtained fractions was determined, revealing higher values in those fractions in which compounds such as gallic acid, protocatechuic acid, protocatechualdehyde, trans-caftaric acid, syringic acid, isovanillin or tyrosol, among others, were present. This is the first time that HSCCC has been used to characterize the phenolic composition of Sherry wines.
PB MDPI
SN 2304-8158
YR 2021
FD 2021-01
LK http://hdl.handle.net/10498/24520
UL http://hdl.handle.net/10498/24520
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 13-nov-2026