RT journal article
T1 Cytogenomics Unveil Possible Transposable Elements Driving Rearrangements in Chromosomes 2 and 4 of Solea senegalensis
A1 Rodríguez Jiménez, María Esther
A1 Cross Pacheco, Ismael
A1 Arias Pérez, Alberto
A1 Portela Bens, Silvia
A1 Merlo Torres, Manuel Alejandro
A1 Liehr, Thomas
A1 Rebordinos González, Laureana
A2 BiomedicinaBiotecnología y Salud Pública
K1 Solea senegalensis
K1 cytogenomics
K1 chromosome evolution
K1 comparative genomic
K1 repetitive sequences
K1 robertsonian fusions
K1 pleuronectiformes
AB Cytogenomics, the integration of cytogenetic and genomic data, has been used here to reconstruct the evolution of chromosomes 2 and 4 of Solea senegalensis. S. senegalensis is a flat fish with a karyotype comprising 2n = 42 chromosomes: 6 metacentric + 4 submetacentric + 8 subtelocentric + 24 telocentric. The Fluorescence in situ Hybridization with Bacterial Artificial Chromosomes (FISH-BAC) technique was applied to locate BACs in these chromosomes (11 and 10 BACs in chromosomes 2 and 4, respectively) and to generate integrated maps. Synteny analysis, taking eight reference fish species (Cynoglossus semilaevis, Scophthalmus maximus, Sparus aurata, Gasterosteus aculeatus, Xiphophorus maculatus, Oryzias latipes, Danio rerio, and Lepisosteus oculatus) for comparison, showed that the BACs of these two chromosomes of S. senegalensis were mainly distributed in two principal chromosomes in the reference species. Transposable Elements (TE) analysis showed significant differences between the two chromosomes, in terms of number of loci per Mb and coverage, and the class of TE (I or II) present. Analysis of TE divergence in chromosomes 2 and 4 compared to their syntenic regions in four reference fish species (C. semilaevis, S. maximus, O. latipes, and D. rerio) revealed differences in their age of activity compared with those species but less notable differences between the two chromosomes. Differences were also observed in peaks of divergence and coverage of TE families for all reference species even in those close to S. senegalensis, like S. maximus and C. semilaevis. Considered together, chromosomes 2 and 4 have evolved by Robertsonian fusions, pericentric inversions, and other chromosomal rearrangements mediated by TEs.
PB MDPI
SN 1422-0067
YR 2021
FD 2021-02
LK http://hdl.handle.net/10498/24655
UL http://hdl.handle.net/10498/24655
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 11-nov-2026