RT journal article
T1 Evolution of Experimental Models in the Study of Glioblastoma: Toward Finding Efficient Treatments
A1 Gómez Oliva, Ricardo
A1 Domínguez García, Samuel
A1 Carrascal, Livia
A1 Abalos Martínez, Jessica
A1 Pardillo Díaz, Ricardo
A1 Verástegui Escolano, Cristina
A1 Castro González, Carmen
A1 Nunez Abades, Pedro
A1 Geribaldi Doldán, Noelia
A2 Anatomía y Embriología Humana
A2 BiomedicinaBiotecnología y Salud Pública
K1 glioma stem cells
K1 cell cultures of glioma cells
K1 mouse models of glioblastoma
K1 brain organoids
K1 3D bioprinting
AB Glioblastoma (GBM) is the most common form of brain tumor characterized by its resistance to conventional therapies, including temozolomide, the most widely used chemotherapeutic agent in the treatment of GBM. Within the tumor, the presence of glioma stem cells (GSC) seems to be the reason for drug resistance. The discovery of GSC has boosted the search for new experimental models to study GBM, which allow the development of new GBM treatments targeting these cells. In here, we describe different strategies currently in use to study GBM. Initial GBM investigations were focused in the development of xenograft assays. Thereafter, techniques advanced to dissociate tumor cells into single-cell suspensions, which generate aggregates referred to as neurospheres, thus facilitating their selective expansion. Concomitantly, the finding of genes involved in the initiation and progression of GBM tumors, led to the generation of mice models for the GBM. The latest advances have been the use of GBM organoids or 3D-bioprinted mini-brains. 3D bio-printing mimics tissue cytoarchitecture by combining different types of cells interacting with each other and with extracellular matrix components. These in vivo models faithfully replicate human diseases in which the effect of new drugs can easily be tested. Based on recent data from human glioblastoma, this review critically evaluates the different experimental models used in the study of GB, including cell cultures, mouse models, brain organoids, and 3D bioprinting focusing in the advantages and disadvantages of each approach to understand the mechanisms involved in the progression and treatment response of this devastating disease.
PB FRONTIERS MEDIA SA
SN 2234-943X
YR 2021
FD 2021-01
LK http://hdl.handle.net/10498/24667
UL http://hdl.handle.net/10498/24667
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 07-nov-2026