RT journal article
T1 Flavonol Composition and Antioxidant Activity of Onions (Allium cepa L.) Based on the Development of New Analytical Ultrasound-Assisted Extraction Methods
A1 Velasco González de Peredo, Ana
A1 Vázquez Espinosa, Mercedes
A1 Espada Bellido, Estrella
A1 Carrera Fernández, Ceferino Adrián
A1 Ferreiro González, Marta
A1 Fernández Barbero, Gerardo
A1 Palma Lovillo, Miguel
A2 Química Analítica
K1 Allium cepa L
K1 antioxidant activity
K1 Box&#8211
K1 Behnken
K1 flavonoids
K1 quercetin derivatives
K1 multiresponse optimization
K1 onion
K1 phenolic compounds
K1 UHPLC
K1 ultrasound-assisted extraction
AB The onion is one of the most cultivated and consumed vegetables, and is a very valuable source of antioxidant substances. Every onion variety is rich in flavonols-mainly quercetin derivatives which makes onions the main dietary source of these compounds. This study intends to develop an ultrasound-assisted extraction method (UAE), an economical, green, and efficient technique, that allows us to determine the quality of onion extracts in terms of flavonol composition and antioxidant activity. For this purpose, an ultrasound-assisted extraction method has been optimized to obtain extracts with a high amount of flavonols, as well as with a high antioxidant activity, not only separately using a Box-Behnken design, but also simultaneously, based on multi-response optimization. Multiple response optimization has not only demonstrated an efficacy level similar to that of the individual ones, but it also represents a considerable reduction in terms of cost, time and work. The optimal conditions for simultaneous extractions were determined as follows: 76.8% methanol as an extraction solvent at pH 2 and 58.5 degrees C temperature, 85% amplitude, 0.9 s cycle, and 0.2:13 g:mL sample mass/solvent volume ratio. Furthermore, the developed method exhibited a high precision level and great recoveries in a rather short extraction time (2 min). These results, together with the development of a fast and simple UHPLC analysis method, lead us to consider the developed UAE method as a suitable technique for the extraction of bioactive compounds from onion matrices.
PB MDPI
SN 2076-3921
YR 2021
FD 2021-02
LK http://hdl.handle.net/10498/24696
UL http://hdl.handle.net/10498/24696
LA eng
NO This work has been supported by the project "EQC2018-005135-P" (Equipment for liquid chromatography by means of mass spectrometry and ion chromatography), of the State Subprogram of Research Infrastructures and Technical Scientific Equipment. This research was funded by University of Cadiz, by the INIA (National Institute for Agronomic Research) and FEDER (European Regional Development Fund, within the framework of the Operational Programme under the Investment for Growth 2014-2020) for their financial support (Project RTA2014-00083-C03-03), and by the Ministry of Science and Innovation of Spain (FPU grant AP-2018-03811 to Ana Velasco Gonzalez de Peredo).
DS Repositorio Institucional de la Universidad de Cádiz
RD 05-nov-2026