RT journal article
T1 Development of a Rapid UHPLC-PDA Method for the Simultaneous Quantification of Flavonol Contents in Onions (Allium cepa L.)
A1 Velasco González de Peredo, Ana
A1 Vázquez Espinosa, Mercedes
A1 Carrera Fernández, Ceferino Adrián
A1 Espada Bellido, Estrella
A1 Ferreiro González, Marta
A1 Fernández Barbero, Gerardo
A1 Palma Lovillo, Miguel
A2 Química Analítica
K1 Allium cepa L.
K1 Box-Behnken
K1 flavonoids
K1 quercetin glycosides
K1 liquid chromatography
K1 multiresponse optimization
K1 onion
K1 phenolic compounds
K1 UHPLC
AB Onion, one of the most consumed vegetables in the world, is also known to contain high levels of antioxidant compounds, with protective effects against different degenerative pathologies. Specifically, onion is rich in flavonols, mainly quercetin derivatives, which are compounds with high antioxidant and free radical scavenging power. For this reason, it is of the utmost importance to count on optimal analytical methods that allow for the determination and quantification of these compounds of interest. A rapid ultra-high performance liquid chromatography (UHPLC)-photo-diode array (PDA) method for the separation of the major flavonols in onions was developed using a Box-Behnken design in conjunction with multiresponse optimization on the basis of the desirability function. The conditions that provided a successful separation were 9.9% and 53.2% of phase B at the beginning and at the end of the gradient, respectively; 55 degrees C column working temperature; and 0.6 mL min(-1) flow rate. The complete separation was achieved in less than 2.7 min with excellent chromatographic characteristics. The method was validated, and its high precision, low detection and quantification limits, good linearity, and robustness were confirmed. The correct applicability of the method improves the analysis of the raw material, increasing the quality of onions and its subproducts in terms of bioactive compounds and functional characteristics for consumers.
PB MDPI
SN 1424-8247
YR 2021
FD 2021-04
LK http://hdl.handle.net/10498/24917
UL http://hdl.handle.net/10498/24917
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 05-nov-2026