RT journal article
T1 Optimization of an Ultrasound-Assisted Extraction Method for the Analysis of Major Anthocyanin Content in Erica australis Flowers
A1 Carrera Fernández, Ceferino Adrián
A1 Aliaño González, María José
A1 Rodríguez López, Jaime
A1 Ferreiro González, Marta
A1 Ojeda Copete, Fernando
A1 Fernández Barbero, Gerardo
A1 Palma Lovillo, Miguel
A2 Biología
A2 Química Analítica
K1 flower anthocyanins
K1 Mediterranean heather
K1 ultrasound-assisted extraction
K1 optimization
K1 Box-Behnken design
K1 HPLC-UV-vis
AB Erica australis plants have been used in infusions and folk medicine for years for its diureticand antiseptic properties and even for the treatment of infections. In addition, a recently publishedthorough study on this species has demonstrated its antioxidant, antibiotic, anti-inflammatory,anticarcinogenic and even antitumoral activities. These properties have been associated with thehigh content of anthocyanins in E. australis leaves and flowers. The aim of the present research is tooptimize an ultrasound-assisted extraction methodology for the recovery of the anthocyanins presentin E. australis flowers. For that purpose, a Box Behnken design with response surface methodologywas employed, and the influence of four variables at different values was determined: namely,the composition of the extraction solvents (0–50% MeOH in water), the pH level of those solvents(3–7), the extraction temperature (10–70  C), and the sample:solvent ratio (0.5 g:10 mL–0.5 g:20 mL).UHPLC-UV-vis has been employed to quantify the two major anthocyanins detected in the samples.The extraction optimum conditions for 0.5 g samples were: 20 mL of solvent (50% MeOH:H2O) at5 pH, with a 15 min extraction time at 70  C. A precision study was performed and the intra-dayand inter-day relative standard deviations (RSDs) obtained were 3.31% and 3.52%, respectively. Thedeveloped methodology has been successfully applied to other Erica species to validate the suitabilityof the method for anthocyanin extraction.
PB MDPI
SN 1420-3049
YR 2021
FD 2021-05
LK http://hdl.handle.net/10498/25183
UL http://hdl.handle.net/10498/25183
LA eng
NO This work has been supported by the project "EQC2018-005135-P" (Equipment for liquid chromatography by means of mass spectrometry and ion chromatography) of the State Subprogram of Research Infrastructures and Technical Scientific Equipment.
DS Repositorio Institucional de la Universidad de Cádiz
RD 13-nov-2026