RT journal article
T1 Multi-omic approach to evaluate the response of gilt-head sea bream (Sparus aurata) exposed to the UV filter sulisobenzone
A1 Colás Ruiz, Nieves del Rocio
A1 Ramirez, Gaëlle
A1 Courant, Frédérique
A1 Gomez, Elena
A1 Hampel, Miriam
A1 Lara Martín, Pablo Antonio
A2 Química Física
K1 Metabolomics
K1 Transcriptomics
K1 UV-filter
K1 Fish
K1 Emerging contaminants
AB Sulisobenzone (BP-4) is one of the benzophenone type UVfiltersmost frequently detected in aquatic ecosystems.As a suspected endocrine disrupting compound, scarce information is available yet about other molecular effectsand its mechanismof action. Here, we used an integrated transcriptomic and metabolomic approach to improvethe current understanding on the toxicity of BP-4 towards aquatic species. Gilt-head sea bream individualswereexposed at environmentally relevant concentrations (10 μg L-1) for 22 days. Transcriptomic analysis revealed 371differentially expressed genes in liver while metabolomic analysis identified 123 differentially modulated featuresin plasma and 118 in liver. Integration of transcriptomic and metabolomic data showed disruption of theenergy metabolism(>10 pathways related to the metabolismof amino acids and carbohydrateswere impacted)and lipid metabolism (5 glycerophospholipids and the expression of 3 enzymes were affected), suggesting oxidativestress.We also observed, for the first time in vivo and at environmental relevant concentrations, the disruptionof several enzymes involved in the steroid and thyroid hormones biosynthesis. DNA and RNAsynthesis was also impacted by changes in the purine and pyrimidine metabolisms. Overall, the multiomicworkflow presented here increases the evidence on suspected effects of BP-4 exposure and identifies additionalmodes of action of the compounds that could have been overlooked by using single omic approaches.©2021 The Authors. Published by Elsevier B.V.
PB ELSEVIER
SN 0048-9697
YR 2022
FD 2022-01
LK http://hdl.handle.net/10498/25349
UL http://hdl.handle.net/10498/25349
LA eng
NO This research was supported by the Spanish Ministry of Economy,Industry and Competitiveness (MINECO) (CTM2015-70731-R) and the FPI fellowship (BES-2016-078593). The authors also would like to acknowledgethe Laboratory of Aquaculture at the Faculty of Marine andEnvironmental Sciences (University of Cádiz), Thibaut Dumas and DavidRosain (UMR HydroSciences, Université Montpellier, France) for theirsupport.
DS Repositorio Institucional de la Universidad de Cádiz
RD 21-sep-2026