RT journal article
T1 Effect of Washing Treatment on the Textural Properties and Bioactivity of Silica/Chitosan/TCP Xerogels for Bone Regeneration
A1 Pérez Moreno, Antonio
A1 Reyes Peces, María Virtudes
A1 Vilches Pérez, José Ignacio
A1 Fernández Montesinos, Rafael
A1 Pinaglia Tobaruela, Gonzalo
A1 Salido Peracaula, Mercedes
A1 Rosa Fox, Nicolás Daniel de la
A1 Piñero de los Ríos, Manuel
A2 Anatomía PatológicaBiología Celular, Histología, Historia de la Ciencia, Medicina Legal y Forense y Toxicología
A2 Física de la Materia Condensada
A2 Materno-Infantil y Radiología
K1 hybrid xerogels
K1 chitosan
K1 tricalcium phosphate
K1 N2 physisorption
K1 t-plot
K1 bioactivity
K1 biodegradation
K1 bone tissue engineering
K1 osteoblasts
K1 focal adhesions
AB Silica (SiO2)/chitosan (CS) composite aerogels are bioactive when they are submerged in simulated body fluid (SBF), causing the formation of bone-like hydroxyapatite (HAp) layer. Silica-based hybrid aerogels improve the elastic behavior, and the combined CS modifies the network entanglement as a crosslinking biopolymer. Tetraethoxysilane (TEOS)/CS is used as network precursors by employing a sol-gel method assisted with high power ultrasound (600 W). Upon gelation and aging, gels are dried in supercritical CO2 to obtain monoliths. Thermograms provide information about the condensation of the remaining hydroxyl groups (400-700 degrees C). This step permits the evaluation of the hydroxyl group's content of 2 to 5 OH nm(-2). The formed Si-OH groups act as the inductor of apatite crystal nucleation in SBF. The N-2 physisorption isotherms show a hysteresis loop of type H3, characteristic to good interconnected porosity, which facilitates both the bioactivity and the adhesion of osteoblasts cells. After two weeks of immersion in SBF, a layer of HAp microcrystals develops on the surface with a stoichiometric Ca/P molar ratio of 1.67 with spherulite morphology and uniform sizes of 6 mu m. This fact asserts the bioactive behavior of these hybrid aerogels. Osteoblasts are cultured on the selected samples and immunolabeled for cytoskeletal and focal adhesion expression related to scaffold nanostructure and composition. The initial osteoconductive response observes points to a great potential of tissue engineering for the designed composite aerogels.
PB MDPI
SN 1422-0067
YR 2021
FD 2021-08
LK http://hdl.handle.net/10498/25679
UL http://hdl.handle.net/10498/25679
LA eng
NO This research was 80% supported by Andalucia FEDER/ITI 2014-2020 Grant for PI 013/017 and Junta de Andalucia TEP115 and CTS 253 PAIDI research groups (Spain). The work has also been co-financed by the 2014-2020 ERDF Operational Programme and by the Department of Economy, Knowledge, Business and University of the Regional Government of Andalusia. Project reference: FEDER-UCA18_106598.
DS Repositorio Institucional de la Universidad de Cádiz
RD 08-nov-2026