RT journal article
T1 Optimization of the Microwave-Assisted Extraction of Simple Phenolic Compounds from Grape Skins and Seeds
A1 Azaroual, Latifa
A1 Liazid, Ali
A1 El Mansouri, Fouad
A1 Brigui, Jamal
A1 Ruiz Rodríguez, Ana
A1 Fernández Barbero, Gerardo
A1 Palma Lovillo, Miguel
A2 Química Analítica
K1 phenolic compounds
K1 grapes
K1 microwave-assisted extraction
AB A method for the extraction of phenolic compounds from grape seeds and skins using microwave-assisted extraction (MAE) was developed. Optimization of the effects of the extraction parameters in terms of the results of extraction was obtained using the response surface methodology. The parameters studied were extraction solvent (methanol, ethanol, acetone, and water), percentage of methanol in water, quantity of sample in relation to volume of extraction solvent (solid:liquid, 10-50 mg mL(-1)), power (100-500 W), magnetic stirring speed (0-100%), and extraction time (5-20 min). Finally, the repeatability and the intermediate precision of the method were determined. The best conditions proved to be: 65% methanol in water as an optimum extraction solvent; 0.5 g of grape skin or seed in a volume of 25 mL; a power of 500 W with the maximum stirring speed (100%); and an extraction time of 5 min. The phenolic compounds proved to be stable in the optimized extraction conditions. The resulting repeatability and the intermediate precision of the optimized method showed a relative standard deviation below 7%. The new method applied on Napoleon grape allowed for the determination of catechin (453.2 (mg kg(-1))), epicatechin (306.3 mg kg(-1)), caftaric acid (22.37 mg caffeic acid equivalents kg(-1)), dihydrokaempferol-glycoside (11.13 mg kaempferol equivalents kg(-1)), quercetin (18.28 mg kg(-1)), quercetin-3-glucoside (20.09 mg quercetin equivalents kg(-1)), and kaempferol-3-glucoside (11.10 mg kaempferol equivalents kg(-1)).
PB MDPI
SN 2073-4395
YR 2021
FD 2021-08
LK http://hdl.handle.net/10498/25738
UL http://hdl.handle.net/10498/25738
LA eng
NO This research was co-financed by the 2014-2020 ERDF Operational Programme and by the Department of Economy, Knowledge, and Business at the University of the Regional Government of Andalusia. Project reference: FEDER-UCA18-108366.Document
DS Repositorio Institucional de la Universidad de Cádiz
RD 08-nov-2026