RT journal article
T1 The long-term failure of RYGB surgery in improving T2DM is related to hyperinsulinism.
A1 Pérez Arana, Gonzalo Martín
A1 Díaz Gómez, Alfredo Luis
A1 Bancalero-de los Reyes, José
A1 Camacho Ramírez, Alonso
A1 Fernández Vivero, José
A1 Ribelles García, Antonio
A1 Almorza Gomar, David
A1 Carrasco Molinillo, María del Carmen
A1 Mateo-Gavira, Isabel
A1 Prada Oliveira, José Arturo
A2 Anatomía y Embriología Humana
A2 Estadística e Investigación Operativa
K1 Alpha-Cells
K1 Beta-Cell mass
K1 Pancreas
K1 Type 2 Diabetes mellitus
K1 Roux-en Y-gastric bypass
K1 Incretins
K1 Cellular differentiation
AB BACKGROUND: Roux-en-Y gastric bypass (RYGB) is the gold standard method for bariatric surgery and leads to substantial improvements in Type 2 Diabetes mellitus. However, many patients experience relapses in diabetes five years after undergoing this aggressive surgical procedure. We focus on beta-cell population changes and absorptive intestinal consequences after RYGB in a healthy nonobese animal model after a long survival period.METHODS: For our purpose, we use three groups of Wistar rats: RYGB-operated, surgical control (Sham) and fasting control. We measure alpha-, beta-cell mass; transcription (Arx, and Pdx-1) and proliferation (Ki67) factors; glucose tolerance and insulin release after oral glucose tests; histological adaptive changes in the jejunum; and intestinal glucose transporters.RESULTS: Our results showed an early increase in insulin secretion after surgery, that decrease at the end of the study. The beta-cell mass reduces twenty-four weeks after RYGB, which coincides with decrease of Pdx-1 transcription promoter factor. These was coincident with an increase in alpha-mass and a high expression of Arx in RYGB group.CONCLUSIONS: The analysis of all data showed beta-cell mass transdifferentiation into alpha-cell mass in RYGB rats. Due to long-term exhaustion of the beta-cell population by hyperinsulinism derived from digestive tract adaptation to surgery.Copyright © 2021 The Author(s). Published by Elsevier GmbH.. All rights reserved.
PB ELSEVIER
SN 1618-0402
YR 2021
FD 2021-11
LK http://hdl.handle.net/10498/25823
UL http://hdl.handle.net/10498/25823
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 11-nov-2026