RT journal article
T1 Deletion of the Bcnrps1 Gene Increases the Pathogenicity of Botrytis cinerea and Reduces Its Tolerance to the Exogenous Toxic Substances Spermidine and Pyrimethanil
A1 Fernández Morales, Ana
A1 Carbú Espinosa de los Monteros, María
A1 González Rodríguez, Victoria Eugenia
A1 Papaspyrou, Sokratis
A1 Garrido Crespo, Carlos
A1 Cantoral Fernández, Jesús Manuel
A2 Biología
A2 BiomedicinaBiotecnología y Salud Pública
K1 non-ribosomal peptide synthetase
K1 environmental signals
K1 polyamines
K1 fungicide
K1 RTqPCR
K1 siderophore
K1 oxidative stress
AB During the infection of grapevine (Vitis vinifera) by the fungus Botrytis cinerea, the concentrationof polyamines, which are toxic substances for the phytopathogen, increases in the grape.Nine NRPS genes have been identified in the genome of B. cinerea, yet the function of five of themremains unknown. For this reason, we have studied the expression of the 9 NRPS genes by RT-qPCRin a medium supplemented with sublethal concentrations of three polyamines (1,3-diaminopropane(1,3-DAP), spermidine (SPD), and spermine (SPM)). Our results show that the presence of polyaminesin the culture medium triggered the overexpression of the Bcnrps1 gene in the pathogen. Deleting Bcnrps1did not affect mycelial growth or adaptation to osmotic stress, and we show that its expression isnot essential for the cycle of infection of the B. cinerea. However, mutating the Bcnrps1 gene resulted inoverexpression of the Bcnrps6 gene, which encodes for the excretion of siderophores of the coprogenfamily. Moreover, gene deletion has reduced the tolerance of B. cinerea B05.10 to toxic substances suchas the polyamine SPD and the fungicide pyrimethanil, and its virulence has increased. Our findingsprovide new insights into the function of the Bcnrps1 gene and its involvement in the tolerance ofB. cinerea against exogenous toxic compounds.
PB MDPI
SN 2309-608X
YR 2021
FD 2021-09
LK http://hdl.handle.net/10498/25831
UL http://hdl.handle.net/10498/25831
LA eng
NO This work was supported by the Spanish Government and European Union, MINECOFEDER, RTI2018-097356-B-C22 and MINECO, AGL2015-65684-C2-2-R.
DS Repositorio Institucional de la Universidad de Cádiz
RD 04-nov-2026